Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea : Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori
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概要
- 論文の詳細を見る
Superoxide dismutase (SOD) is a regulatory enzyme involved in the degradation of superoxide anions in living organisms. In this study, we examined SOD from the fall webworm, Hyphantria cunea (hcSOD). A cDNA encoding hcSOD was cloned by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of hcSOD revealed that six of seven highly conserved residues forming the Cu/Zn-binding sites were present. The hcSOD mRNA and the enzyme activity were distributed in larval tissues including fat body, midgut and hemocyte of H. cunea. A recombinant hcSOD (rhcSOD) functionally overexpressed in Escherichia coli in a soluble form that was purified to homogeneity. It was stable at pHs between 5 and 11. Incubation for 30min at temperatures below 50℃ scarcely affected the activity. All these properties, as well as CD spectra, of hcSOD or rhcSOD were highly similar to those of the Bombyx mori counterparts except for the number of Cu/Zn-binding sites and the specific activity dismutating superoxide anion to peroxide and oxygen.
- 日本応用動物昆虫学会の論文
- 2007-08-25
著者
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Yamamoto K
Kyushu Univ. Fukuoka Jpn
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Koga K
Laboratory Of Silkworm Sciences Faculty Of Agriculture Kyushu University Graduate School
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Koga K
Faculty Of Agriculture Kyushu University Graduate School:(present Office)faculty Of Engineering Kyus
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Koga Katsumi
Laboratory Of Sericulture Faculty Of Agriculture Kyushu University Fukuoka
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Banno Y
Kyushu Univ. Fukuoka Jpn
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Banno Yutaka
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Yamamoto K
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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KIMURA Makoto
Faculty of Agriculture, The University of Tokyo
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Yamamoto K
Kyushu Univ. Fukuoka
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Yamamoto Kohji
Laboratory Of Soil Science And Plant Nutrition College Of Agriculture:(present Address)wakayama Pref
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Yamamoto Kohji
Laboratory Of Insect Genetic Resources Division Of Applied Genetic Resources Department Of Genetic R
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ASO Yoichi
Faculty of Agriculture, Kyushu University Graduate School
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KOGA Katsumi
Faculty of Agriculture, Kyushu University Graduate School
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Aso Y
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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Kimura Makoto
Faculty Of Agriculture Kyushu University Graduate School
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ASO Yoichi
Faculty of Agriculture, Kyushu University
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