Potassium Iodide-Induced Changes in Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus
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概要
- 論文の詳細を見る
Treatment with KI and its subsequent removal induced disassembly of Bacillus stearotheimophilus pyruvate dehydrogenase complex (PDC) and association of disassembly products, respectively. The disassembly yielded neither completely dissociated components nor aggregate, but did yield a few molecular forms smaller than PDC. Depending on the KI concentration, these changes were of three phases: K-1, below 0.6 M; K-2, 1.0-1.5 M; K-3, above 1.8M. PDC was disassembled in K-1 to C1 comprising pyruvate decarboxylase and lipoate acetyltransferase mainly and C2 comprising the decarboxylase and dihydrolipoamide dehydrogenase. In K-1, the removal of KI resulted in an apparent reconstitution of PDC. The mixing of Cl with an excess of C2 yielded an assembly larger than PDC and restored enzyme activities, but specific activities were different from those of PDC. In K-2 and K-3 phases, complexes smaller than PDC were yielded from disassembly products, and activities except for that of the acetyltransferase were not restored.
- 1998-01-23
著者
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MENO Kohji
Laboratory of Protein Chemistry & Engineering, Kyushu University
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ASO yoichi
Laboratory of Protein Chemistry & Engineering, Kyushu University
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Aso Y
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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Meno Kohji
Laboratory Of Protein Chemistry And Engineering Department Of Genetic Resources Technology Graduate
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Ishiguro M
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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AIKAWA Yoshikatsu
Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Ky
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Ishiguro M
Kyushu Univ. Fukuoka Jpn
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Ishiguro Masatsune
Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr
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HIROMASA Yasuaki
Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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Aikawa Yoshikatsu
Laboratory Of Protein Chemistry And Engineering Graduate School Of Genetic Resources Technology Kyus
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Hiromasa Yasuaki
Laboratory Of Protein Chemistry And Engineering Department Of Genetic Resources Technology Graduate
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Ishiguro Masatsune
Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
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ASO Yoichi
Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University
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