Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• In order to understand a physiological role of chitinases in rye, the localization and accumulation of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by immunochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), which is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot analysis detected both RSC-a and RSC-c in the endosperm of the rye seed. Immunohistochemical staining indicated that RSC-a was localized in only the aleurone cells, whereas RSC-c existed at least in the starchy endosperm and was also likely to exist in the aleurone cells. It was found by ELISA and an immunoblot analysis that RSC-a and -c accumulated in the seed during the later stage of development. Both chitinases and the Cat-domain exhibited antifungal activity toward Trichoderma species, while the CB-domain did not. Observation of the inhibition of hyphal growth of the T. species suggests that the two chitinases acted in different ways.

• 社団法人日本農芸化学会の論文
• 2001-12-23

著者

• Yanagita T

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• ASO yoichi

  Laboratory of Protein Chemistry & Engineering, Kyushu University

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Yanagita Teruyoshi

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• Aso Y

  Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• TAIRA Toki

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

• ISHIHARA Masanobu

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

• Ishihara M

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Ishihara Masanobu

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Yamagami T

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Taira Toki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Taira T

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

• ASO Yoichi

  Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

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