Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
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概要
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A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatography, hydrophobic chromatography on Butyl Toyopearl 650S, and gel filtration on Toyopearl HW55. The molecular weights (Mr) of FAE-a and FAE-b were estimated to be 31 KDa and 29 KDa by SDS-PAGE; these isoelectric points were 3.9 and below 3.4, respectively. Both enzymes were optimally active at 45-50℃ and pH 5.0-5.5. FAE-a was stable up to 37℃, and FAE-b was stable up to 50℃. Both enzymes hydrolyzed predominantly the feruloylated carbohydrate ester prepared from pineapple stem residue rather than methyl esters of ferulic acid.
- 社団法人 日本食品科学工学会の論文
- 1999-08-01
著者
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Nakazato Naoko
Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus
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Ishihara M
Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci
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Ishihara Masanobu
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
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Toyama Seizen
Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus
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Tawata S
Univ. Ryukyus Okinawa Jpn
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Tawata Shinkichi
Department Of Bioscience And Biotechnology College Of Agriculture University Of Ryukyus
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CHIBANA Kunio
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus
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Chibana Kunio
Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus
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TOYAMA Seizen
Department of Agricultural Chemistry, University of the Ryukyus
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