Angiotensin I-Converting Enzyme Inhibitory Peptides Isolated from Tofuyo Fermented Soybean Food(Food & Nutrition Science)

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概要

• 論文の詳細を見る

• Angiotensin I-converting enzyme (ACE) inhibitory activity was observed in a tofuyo (fermented soybean food) extract with an IC_<50> value of 1.77 mg/ml. Two ACE inhibitors were isolated to homogeneity from the extract by adsorption and gel filtration column chromatography, and by reverse-phase high-performance liquid chromatography (HPLC). The purified substances reacted with 2, 4, 6-trinitrobenzensulfonic acid sodium salt. The amino acid sequences of these inhibitors determined by Edman degradation were Ile-Phe-Leu (IC_<50>, 44.8μM) and Trp-Leu (IC_<50>, 29.9 μM). The Ile-Phe-Leu sequence is found in the α- and β-subunits of β-conglycinin, while the Trp-Leu sequence is in the B-, B1A- and BX-subunits of glycinin from soybean. Both of the peptides are non-competitive inhibitors. The inhibitory activity of Trp-Leu was completely preserved after a treatment with pepsin, chymotrypsin or trypsin. Even after successive digestion by these gastrointestinal proteases, the activity remained at 29% of the original value.

• 社団法人日本農芸化学会の論文
• 2003-06-23

著者

• Takeda Yasuhito

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• TAWATA Shinkichi

  Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus

• Tanaka Kumi

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus

• Yasuda Masaaki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus

• Tawata Shinkichi

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus

• Tawata Shinkichi

  Department Of Bioscience And Biotechnology College Of Agriculture University Of Ryukyus

• KUBA Megumi

  Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus

• Kuba M

  Univ. The Ryukyus Okinawa Jpn

• Yasuda Masaaki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of Ryukyus

• YASUDA Masaaki

  Department of Agricultural Chemistry, University of the Ryukyus

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