Major Isoforms of Starch Branching Enzymes in Premature Seeds of Kidney Bean (Phaseolus vulgaris L.)
スポンサーリンク
概要
- 論文の詳細を見る
Developing seeds of the kidney bean (Phaseolus vulgaris L.) contain several isoforms of starch branching enzymes. Two of them, KBE1 and KBE2,which are the major forms in the premature seeds, were purified as a single band of protein on SDS-PAGE and native PAGE by chromatographies on DEAE-Sepharose, Bio-Gel P-200,and amylose-binding Sepharose 6B. The enzymes had similar pH optimum (7.0), pH stability (7.0-9.5), temperature optimum (25-30℃), and temperature stability (up to 40℃). Additionally, both were inhibited by various divalent metal ions and activated by citrate. Finally, though their N-terminal amino acid sequences were identical, their molecular masses and affinities for amylose differed ; 80kDa and 1.27mM for KBE1 and 77 kDa and 0.74 mM for KBE2.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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Ito Hiroyuki
Department Of Internal Medicine Division Of Cardiovascular And Respiratory Medicine Akita University
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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HONMA Mamoru
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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NOZAKI Kouichi
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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HAMADA Shigeki
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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NAKAMORI Tomoko
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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SAGISAKA Shonosuke
Institute for Plant Cytochemistry, Tonden
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YOSHIDA Hironori
Department of Biochemical Science and Technology, Kagoshima University
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TAKEDA Yasuhito
Department of Biochemical Science and Technology, Kagoshima University
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Ito Hiroyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Nakamori Tomoko
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Takeda Yasuhito
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Takeda Yasuhiro
Department Of Animal Science Utsunomiya University
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Yoshida Hironori
Department Of Biochemical Science And Technology Kagoshima University
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma Mamoru
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Hiraga Susumu
Department Of Applied Biosciences Graduate School Of Agriculture Hokkaido University
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Nozaki K
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University:(present Address
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Ito H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ito Hiroyuki
Department Of Cardiology Akita University
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Hamada Shigeki
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Sagisaka Shonosuke
Institute For Plant Cytochemistry Tonden
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Ito Hiroyuki
Department Of Anesthesiology Tokyo Medical And Dental University Graduate School Of Medical And Dent
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MATSUI Hirokazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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