Production of Lupin Acid Phosphatase in Transgenic Rice for Use as a Phytate-hydrolyzing Enzyme in Animal Feed

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概要

• 論文の詳細を見る

• The acid phosphatase gene from lupin was expressed in transgenic rice plants under the control of the maize ubiquitin promoter or rice chlorophyll a/b binding protein (Cab) promoter. Transgenic rice leaves exhibited up to an 18-fold increase in phytate-hydrolyzing activity. Based on the phytate-hydrolyzing activity at pH 5.5, more than 85% this activity was retained after heat-treatment at 80 °C for 15 min, and the heterologous enzyme in leaf sections and leaf extracts was relatively stable during storage. A distinct increase in released phosphate was observed when the heterologous enzyme was mixed with the feed extract. These results suggest that the heterologous enzyme in rice plants may maintain its desired characteristics as a phytate-hydrolyzing enzyme when added to animal feed.

• 社団法人 日本農芸化学会の論文
• 2004-07-23

著者

• OSAKI Mitsuru

  Graduate School of Agriculture, Hokkaido University

• Shinano Takuro

  Creative Research Institute "Sousei" (CRIS), Hokkaido University

• 大崎 満

  北海道大学農学部

• Wasaki Jun

  Creative Res. Initiative Sousei (cris) Hokkaido Univ.

• Wasaki Jun

  Creative Research Initiative "sousei" Hokkaido University

• 大崎 満

  北大 農

• HAMADA Akira

  Functional Chemicals Laboratory, Mitsui Chemicals, Inc.

• YAMAGUCHI Ken-ichi

  Functional Chemicals Laboratory, Mitsui Chemicals, Inc.

• HARADA Michiko

  Functional Chemicals Laboratory, Mitsui Chemicals, Inc.

• NIKUMARU Seiya

  Functional Chemicals Laboratory, Mitsui Chemicals, Inc.

• MATSUI Hirokazu

  Graduate School of Agriculture, Hokkaido University

• HONDA Hideo

  Functional Chemicals Laboratory, Mitsui Chemicals, Inc.

• Matsui Hirokazu

  Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University

• Honda Hideo

  Functional Chemicals Laboratory Mitsui Chemicals Inc.

• Omura Masanori

  Graduate School Of Agriculture Hokkaido University

• Shinano Takuro

  Creative Research Initiative "sousei" (cris) Hokkaido University

• Harada Michiko

  Functional Chemicals Laboratory Mitsui Chemicals Inc.

• Nikumaru Seiya

  Functional Chemicals Laboratory Mitsui Chemicals Inc.

• Matsui H

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Shinano Takuro

  National Agricultural Res. Center For Hokkaido Region.

• Yamaguchi Ken-ichi

  Faculty Of Horticulture Minami Kyushu University

• Yamaguchi Kenichi

  Faculty Of Horticulture Minami Kyushu University

• Shinano T

  National Agricultural Research Center For Hokkaido Region

• Osaki Mitsuru

  Graduate School Of Agriculture Hokkaido Univ.

• MATSUURA Hideyuki

  Research Faculty of Agriculture, Hokkaido University

• Matsui Hirokazu

  Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University

• Wasaki Jun

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Hamada Akira

  Functional Chemicals Laboratory Mitsui Chemicals Inc.

• Matsui Hirokazu

  Lab. Of Biochemistry Div. Of Applied Bioscience Res. Fac. For Agriculture Hokkaido Univ.

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