Production of Lupin Acid Phosphatase in Transgenic Rice for Use as a Phytate-hydrolyzing Enzyme in Animal Feed
スポンサーリンク
概要
- 論文の詳細を見る
The acid phosphatase gene from lupin was expressed in transgenic rice plants under the control of the maize ubiquitin promoter or rice chlorophyll a/b binding protein (Cab) promoter. Transgenic rice leaves exhibited up to an 18-fold increase in phytate-hydrolyzing activity. Based on the phytate-hydrolyzing activity at pH 5.5, more than 85% this activity was retained after heat-treatment at 80 °C for 15 min, and the heterologous enzyme in leaf sections and leaf extracts was relatively stable during storage. A distinct increase in released phosphate was observed when the heterologous enzyme was mixed with the feed extract. These results suggest that the heterologous enzyme in rice plants may maintain its desired characteristics as a phytate-hydrolyzing enzyme when added to animal feed.
- 社団法人 日本農芸化学会の論文
- 2004-07-23
著者
-
OSAKI Mitsuru
Graduate School of Agriculture, Hokkaido University
-
Shinano Takuro
Creative Research Institute "Sousei" (CRIS), Hokkaido University
-
大崎 満
北海道大学農学部
-
Wasaki Jun
Creative Res. Initiative Sousei (cris) Hokkaido Univ.
-
Wasaki Jun
Creative Research Initiative "sousei" Hokkaido University
-
大崎 満
北大 農
-
HAMADA Akira
Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
-
YAMAGUCHI Ken-ichi
Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
-
HARADA Michiko
Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
-
NIKUMARU Seiya
Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
-
MATSUI Hirokazu
Graduate School of Agriculture, Hokkaido University
-
HONDA Hideo
Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
-
Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
-
Honda Hideo
Functional Chemicals Laboratory Mitsui Chemicals Inc.
-
Omura Masanori
Graduate School Of Agriculture Hokkaido University
-
Shinano Takuro
Creative Research Initiative "sousei" (cris) Hokkaido University
-
Harada Michiko
Functional Chemicals Laboratory Mitsui Chemicals Inc.
-
Nikumaru Seiya
Functional Chemicals Laboratory Mitsui Chemicals Inc.
-
Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
-
Shinano Takuro
National Agricultural Res. Center For Hokkaido Region.
-
Yamaguchi Ken-ichi
Faculty Of Horticulture Minami Kyushu University
-
Yamaguchi Kenichi
Faculty Of Horticulture Minami Kyushu University
-
Shinano T
National Agricultural Research Center For Hokkaido Region
-
Osaki Mitsuru
Graduate School Of Agriculture Hokkaido Univ.
-
MATSUURA Hideyuki
Research Faculty of Agriculture, Hokkaido University
-
Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
-
Wasaki Jun
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Hamada Akira
Functional Chemicals Laboratory Mitsui Chemicals Inc.
-
Matsui Hirokazu
Lab. Of Biochemistry Div. Of Applied Bioscience Res. Fac. For Agriculture Hokkaido Univ.
関連論文
- 6 カドミウム耐性を有する微生物による植物生長への影響(北海道支部講演会,2007年度各支部会)
- 8-22 アーバスキュラー菌根菌におけるポリリン酸代謝 : 潜在的蓄積能とその電荷バランス調節メカニズム(8.共生,2009年度京都大会)
- Influence of arsenic stress on synthesis and localization of low-molecular-weight thiols in Pteris vittata
- Neutral rhizoplane pH of local rice and some predominant tree species in South and Central Kalimantans : a possible strategy of plant adaptation to acidic-soil
- 11 無菌条件下におけるイノシン施与が根の生長に与える影響(北海道支部講演会,2008年度各支部会)
- P20-1 イネ科植物における有機質資材からの窒素獲得(ポスター紹介,20.土壌改良資材,2009年度京都大会)
- P11-14 モエジマシダのヒ素耐性メカニズム : チオール化合物の寄与(ポスター紹介,11.植物の有害元素,2009年度京都大会)
- 9-16 シロバナルーピン由来酸性ホスファターゼ及びフィターゼ過剰発現タバコにおける土壌中有機態リンの利用(9.植物の多量栄養素,2009年度京都大会)
- 01-012 熱帯性植物Melastoma malabathricum L.根圏における窒素固定細菌の群集構造解析(土壌生態系,研究発表)
- P20-4 ホウレンソウのヒ素及びカドミウム吸収に対する零価鉄施与の効果(ポスター紹介,20.土壌改良資材,2008年度愛知大会)
- 11-10 根圏プロテアーゼに着目した、植物の有機態窒素利用の検討(11.植物の栄養生態,2008年度愛知大会)
- 9-45 シロバナルーピン由来酸性ホスファターゼアイソザイムの発現とプロモーター領域の解析(9.植物の無機栄養,2008年度愛知大会)
- 9-27 モエジマシダのヒ素無毒化機構におけるチオールペプチドの役割について(9.植物の無機栄養,2008年度愛知大会)
- 9-1 Ionomics study of Lotus japonicus reveals the element homeostasis network in plants
- Analysis of Diversity of Diazotrophic Bacteria Associated with the Rhizosphere of a Tropical Arbor, Melastoma malabathricum L.
- Element interconnections in Lotus japonicus : A systematic study of the effects of element additions on different natural variants(Plant Nutrition)
- Sago (Metroxylon sagu Rottb.) growth is affected by weeds in a tropical peat swamp in Thailand(Plant Nutrition)
- Screening of phosphate-solubilizing microorganisms in rhizosphere and rhizoplane of adverse soil-adapting plants in Southern Thailand
- Role of Phospho enol pyruvate Carboxylase in the Adaptation of a Tropical Forage Grass to Low-Phosphorus Acid Soils
- The Function of a Maize-Derived Phosphoenolpyruvate Carboxylase (PEPC) in Phosphorus-Deficient Transgenic Rice (Plant Nutrition)
- P12-7 アブラナ科植物に対する堆肥施用効果の代謝産物および遺伝子発現プロファイリング(ポスター紹介,12.農産物の品質・成分,2008年度愛知大会)
- タマネギの根の浸出物による菌根形成の調節(8. 共生, 2004年度大会講演要旨集)
- 8-26 アーバスキュラー菌根菌Glomus spp.におけるマイコウイルスの発見(8.共生,2009年度京都大会)
- P-27 植物根圏を対象としたメタゲノム解析に向けた土壌微生物DNA獲得法の検討(ポスター発表)
- 12 リン酸アルミニウム可溶化能を有する細菌の単離および性状解析(北海道支部講演会,2008年度各支部会)
- 6-12 メタゲノム解析手法を用いた根圏微生物群集によるフィチン酸態リン可給化機構の解析(6.土壌生物,2009年度京都大会)
- 11 イノシンの施用が各種作物の生育におよぼす影響(北海道支部講演会,2007年度各支部会)
- 10 マイクロバブルによる根圏への酸素供給効果の圃場における検証(北海道支部講演会,2007年度各支部会)
- 9 土壌リン形態変化の^P NMRによる解析(北海道支部講演会,2007年度各支部会)
- P9-29 局所リン濃度に応答して発現が変動する遺伝子の解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)
- 8-13 フィチン酸資化性細菌によるクラスター根の形成誘導 : ホワイトルーピンの土壌フィチン酸利用プロセスの解明に向けて(8.共生,2008年度愛知大会)
- S1-3 メタゲノム的アプローチを用いた植物根圏土壌微生物叢の比較解析(シンポジウムI:微生物生態学における環境ゲノミクス,第23回大会シンポジウム)
- 14 Mechanisms on leaf senescence balance related to grain formation in maize
- 11-2 Carbon and nitrogen balance in maize hybrids with different senescence appearance
- Overexpression of the LASAP2 gene for secretory acid phosphatase in white lupin improves the phosphorus uptake and growth of tobacco plants(Plant Nutrition)
- Analysis of bacterial communities on alkaline phosphatase genes in soil supplied with organic matter(Soil Biology)
- Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
- Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH
- Developmental regulation of photosynthate distribution in leaves of rice
- Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability
- Production of Lupin Acid Phosphatase in Transgenic Rice for Use as a Phytate-hydrolyzing Enzyme in Animal Feed
- Low Phosphorus Tolerance Mechanisms: Phosphorus Recycling and Photosynthate Partitioning in the Tropical Forage Grass, Brachiaria Hybrid cultivar Mulato Compared with Rice
- Possibility of Rhizosphere Regulation Using Acid Phosphatase and Organic Acid for Recycling Phosphorus in Sewage Sludge(Plant Nutrition)
- Breeding Wheat for Zinc Efficiency Improvement in Semi-arid Climate-A Review
- Molecular Cloning and Root Specific Expression of Secretory Acid Phosphatase from Phosphate Deficient Lupin (Lupinus albus L.)
- Secreting Portion of Acid Phosphatase in Roots of Lupin (Lupinus albus L.) and a Key Signal for the Secretion from the Roots
- Structure of a cDNA for an Acid Phosphatase from Phosphate-Deficient Lupin (Lupinus albus L.) Roots
- Properties of secretory acid phosphatase from lupin roots under phosphorus-deficient conditions
- 9 有機物施用圃場におけるアルカリホスファターゼ活性とその生成細菌群集の解析(北海道支部講演会,2006年度各支部会)
- 8-16 リン酸施肥はアーバスキュラー菌根菌群集の規定要因となりうるか? : 90年連用圃場を利用したトラップカルチャーによる検証(8.共生,2009年度京都大会)
- Harnessing wheat genotype x Azotobacter strain interactions for sustainable wheat production in semi arid tropics
- 第6部門 : 植物の代謝および代謝成分
- Metabolite profiling of spinach (Spinacia oleracea L.) leaves by altering the ratio of NH_4^+/NO_3^- in the culture solution(Plant Nutrition)
- Localization of acid phosphatase activities in the roots of white lupin plants grown under phosphorus-deficient conditions(Plant Nutrition)
- Frequent isolation of sphingomonads from local rice varieties and other weeds grown on acid sulfate soil in South Kalimantan, Indonesia
- Characterization and ecological role of free-living nitrogen-fixing bacteria isolated from the rhizoplane of Melastoma malabathricum inhabiting acidic plain lands in Kalimantan
- Soil nitrogen supply and nitrogen uptake for local rice grown in unfertilized acid sulfate soil in South Kalimantan
- Phosphorus Solubilizing Microorganisms in the Rhizosphere of Local Rice Varieties Grown without Fertilizer on Acid Sulfate Soils
- Community structures of arbuscular mycorrhizal fungi associated with pioneer grass species Miscanthus sinensis in acid sulfate soils : habitat segregation along pH gradients
- 8-19 Community structures of arbuscular mycorrhizal fungi in the pioneer vegetation of acid sulfate soils : soil pH as a major determinant of community
- 8-4 Isolation and identification of arbuscular mycorrhizal fungi in symbiosis with Miscanthus sinensis in acid sulfate soil
- Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry
- Screening of rhizobacteria from dipterocarp seedlings and saplings for the promotion of early growth of Shorea selanica seedlings
- Ethnobotanical study and nutrient potency of local traditional vegetables in Central Kalimantan
- Promotion of root growth by the application of inosine(Plant Nutrition)
- Amaranthus Tricolor Has the Potential for Phytoremediation of Cadmium-Contaminated Soils
- Effect of Zero-Valent Iron Application on Cadmium Uptake in Rice Plants Grown in Cadmium-Contaminated Soils
- Interacting effects of high temperature and drought stresses in wheat genotypes under semiarid tropics- an appraisal
- Growth promotion of rice by inoculation of acid-tolerant, N_2-fixing bacteria isolated from acid sulfate paddy soil in South Kalimantan, Indonesia
- Wheat improvement under water deficit conditions in semi arid tropics and subtropics
- III-1 植物における光合成産物や窒素の転流・分配と生産能(III.葉の形態形成・機能からみた植物の生産能)
- III-4 酸性環境に対する植物の反応(III.酸性雨の生態系影響の評価)
- ^CO_2トレーサによる植物個体での構造と機能の関係の解析
- Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
- Gas chromatography-mass spectrometry associated global analysis of rice root exudates under aseptical conditions(Plant Nutrition)
- WATERTABLE STUDY OF SAGO PALM ON TROPICAL PEAT
- 23-4 Characteristics of mineral elements in leaves of native plants grown in a distinct ecosystem of tropical peatland in Central Kalimantan, Indonesia
- Identification and characterization of genomic regions associated with nitrogen dynamics in rice plants (Oryza sativa L.)
- Quantitative Trait Loci Affecting Flag Leaf Development in Rice (Oryza sativa L.)
- 熱帯泥炭土壌からのアーバスキュラー菌根菌の分離・増殖とその植物成育への影響(8. 共生, 2004年度大会講演要旨集)
- 9-52 Difference of the mineral characteristics in maize and amaranthus of C_4 plants
- Effect of panicle removal on cytokinin level in the xylem and nitrogen uptake activity of rice
- 11-10 Delayed fluorescence image of leaves in the drought tolerant wheat lines under ambient and elevated CO2 conditions.
- 2 Anti-fungal and plant growth promoting activities in BRF-1, a bacterium isolated from soybean roots
- Difference in Carbon Distribution of Initial Photoassimilates between Soybean and Rice as Revealed by 20 s Pulse-300 s Chase Experiments
- クリーンな紙パルプ製品を得るための菌及びその酵素の利用
- Adaptations of tropical plants to acid soils
- Response of Determinate- and Semi-Determinate-Types of Common Bean to Elevated CO_2 Concentration in the Atmosphere(Plant Nutrition)
- Differences in the Metabolite Profiles of Spinach (Spinacia oleracea L.) Leaf in Different Concentrations of Nitrate in the Culture Solution
- P16-7 有機質肥料適応型トウモロコシ品種・系統の選抜(ポスター紹介,16.畑地土壌肥よく度,2010年度北海道大会)
- Root-Shoot Interaction as a Limiting Factor of Biomass Productivity in New Tropical Rice Lines(Plant Nutrition)
- 11-8 Root activity and biomass productivity of new type of tropical rice plant
- 11-19 Relationships between stem sap exudation and other root characteristics in new plant type of tropical rice
- 11-12 モエジマシダのヒ素耐性における硫黄の重要性(11.植物の有害元素,2010年度北海道大会)
- 19 異なる貧栄養土壌における三種の植物の生育調査(北海道支部講演会,2009年度各支部会)
- 22 ヒ素ストレスに対する植物の代謝応答解析(北海道支部講演会,2009年度各支部会)
- Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
- 16-5 長期継続三要素試験圃場の土壌とそこで栽培したトウモロコシが含む元素の一斉分析(16.畑地土壌肥よく度,2010年度北海道大会)
- Ionomic response of Lotus japonicus to different root-zone temperatures(Plant Nutrition)
- 35 Ionomic response of Lotus japonicusto different root-zone temperatures