Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
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概要
- 論文の詳細を見る
Cellobiose 2-epimerase (CE, EC 5.1.3.11) catalyzes the reversible epimerization of cellobiose to 4-O-β-D-glucopyranosyl-D-mannose. In this study, we found a CE gene in the genome sequence of non-cellulolytic Bacteroides fragilis NCTC 9343. The recombinant enzyme, expressed in Escherichia coli cells, catalyzed a hydroxyl stereoisomerism at the C-2 positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides, lactose, β-mannobiose (4-O-β-D-mannopyranosyl-D-mannose), and globotriose [O-α-D-galactopyranosyl-(1→4)-O-β-D-galactopyranosyl-(1→4)-D-glucose]. The CE from B. fragilis showed less than 40% identity to reported functional CEs. It exhibited 44–63% identities to N-acyl-D-glucosamine 2-epimerase-like hypothetical proteins of unknown function in bacterial genome sequences of the phyla Firmicutes, Bacteroidetes, Proteobacteria, Chloroflexi, and Verrucomicrobia. On the other hand, it showed less than 26% identity to functional N-acyl-D-glucosamine 2-epimerases. Based on the amino acid homology and phylogenetic positions of the functional epimerases, we emphasize that many genes for putative N-acyl-D-glucosamine 2-epimerases and related hypothetical proteins of unknown function reported to date in the bacterial genomes should be annotated as CE-like proteins or putative CEs.
- 社団法人 日本農芸化学会の論文
- 2009-02-23
著者
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Yokota Atsushi
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
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Fukiya Satoru
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
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佐々 武史
山形大農
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WATANABE Jun
Creative Research Initiative Sousei, Hokkaido University
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佐々 武士
山形大学
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Wasaki Jun
Creative Res. Initiative Sousei (cris) Hokkaido Univ.
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ITO Susumu
Creative Research Initiative "Sousei" (CRIS), Hokkaido University
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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森 春英
北海道大学大学院農学研究院応用生命科学部門
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Yokota Atsushi
Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University
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Yokota Atsushi
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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MATSUI Hirokazu
Laboratory of Biochemistry, Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Laboratory Of Biochemistry Department Of Bioscience And Chemistry Faculty Of Agriculture Hokkaido Un
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Senoura Takeshi
Creative Research Initiative Sousei (CRIS), Hokkaido University
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Taguchi Hidenori
Creative Research Initiative Sousei (CRIS), Hokkaido University
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Ito Shigeaki
Laboratory of Biochemistry, Research Faculty of Agriculture, Hokkaido University
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HAMADA Shigeki
Laboratory of Biochemistry, Research Faculty of Agriculture, Hokkaido University
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Hiraga Susumu
Department Of Applied Biosciences Graduate School Of Agriculture Hokkaido University
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Fukiya Satoru
Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University
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Watanabe Jun
Creative Research Initiative Sousei Hokkaido University
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MATSUURA Hideyuki
Research Faculty of Agriculture, Hokkaido University
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Yokota Atsushi
Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ.
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Ito Susumu
Creative Research Initiative Sousei (cris) Hokkaido University
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Ito Shigeaki
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Hamada Shigeki
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Ito Susumu
Creative Res. Initiative Sousei Hokkaido Univ.
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Taguchi Hidenori
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Senoura Takeshi
Creative Research Initiative Sousei (cris) Hokkaido University
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Matsui Hirokazu
Lab. Of Biochemistry Div. Of Applied Bioscience Res. Fac. For Agriculture Hokkaido Univ.
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Watanabe Jun
Creative Res. Initiative ‘sousei' Hokkaido Univ.
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TAGUCHI Hidenori
Research Faculty of Agriculture, Hokkaido University
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