Metabolism of DFA III by Arthrobacter sp. H65-7 : Purification and Properties of a DFA III Hydrolysis Enzyme (DFA IIIase)

スポンサーリンク

概要

• 論文の詳細を見る

• Assimilation of DFA III by Arthrobacter sp. H65-7 was found to consist of two sequential enzyme steps, hydrolysis of DFA III to inulobiose (1-O-β-D-fructofuranosyl-D-fructopyranose) and inulobiose to fructose, that is, x-(2→3') and β-(2'→1) fructosidic linkages were split separately. The enzyme catalyzing the first step, named DFA III hydrolysis enzyme (DFA IIIase), has been purified from the cell-free extracts of Arthrobacter sp. H65-7 to an electrophoretically pure state by heat-treatment, ammonium sulfate fractionation, and chromatographies on DEAE-Toyopearl 650M, Butyl-Sepharose 4B and TSKgel G3000SW_<XL>, and Biophoresis III. The molecular weight of the purified enzyme was estimated to be 125,000 by gel filtration, and 61,000 by SDS-polyacrylamide gel electrophoresis. The enzyme showed the highest activity at pH 6.0 and 45℃, and was stable from pH 4.5 to 10.0 and up to 60℃. The K_m of this enzyme for DFA III was 12.5 mM. The enzyme also catalyzed the reverse reaction, inulobiose to DFA III.

• 社団法人日本農芸化学会の論文
• 1997-06-23

著者

• Sakurai Hiroaki

  Research Center, Nippon Beet Sugar Mfg. Co., Ltd.

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• 横田 篤

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Sakurai Hidetoshi

  Department Of Chemistry And Life Science Nihon University

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• Sakurai Hidetoshi

  Nihon University

• Mori Yumiko

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• SAKURAI Hiroaki

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• SUMITA Yoko

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• MATSUI Hirokazu

  Laboratory of Biochemistry, Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido

• Mori Yuichiro

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Sumita Y

  Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci

• Sakurai H

  Nihon Univ. Fujisawa‐shi Jpn

• Matsui Hirokazu

  Laboratory Of Biochemistry Department Of Bioscience And Chemistry Faculty Of Agriculture Hokkaido Un

• Sumita Yoko

  Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci

• Sakurai Hiroaki

  Research Center Nippon Beet Sugar Mfg. Co. Ltd.

• Sakurai Hiroaki

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

関連論文

• One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-assimilating bacterium Ruminococcus productus on rat intestine.
• Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• rDNA ITS Sequence of Rhizopus oryzae : Its Application to Classification and Identification of Lactic Acid Producers(Microbiology & Fermentation Technology)
• Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces
• Production of Lepidimoide by an Endophytic Fungus from Polysaccharide Extracted from Abelmoschus sp. : Identification of the Product and the Organism Producing It
• Endophytes as Producers of Xylanase
• Isolation, Screening and Phylogenetic Identification of Endophytes from Plants in Hokkaido Japan and Java Indonesia
• 450 有用な植物内生菌の探索とrDNA解析による分類と同定
• 943 糸状菌12-3株によるオクラ多糖からのレピヂモイド生産
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• 543 原油中難分解成分の低温分解菌の探索
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• Development and assessment of a Real-Time PCR assay for the rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces
• Isolation of a Cadmium-releasing Bacterium and Characterization of Its Novel Protease
• Development of Internal Transcribed Spacer Regions Amplification Restriction Fragment Length Polymorphism Methhod and Its Application in Monitoring the Population of Zygosaccharomyces rouxii M2 in Miso Fermentation
• Enhancement of Glucose Metabolism a Pyruvic Acid-Hyperproducing Escherichia coli Mutant Defective in F_1-ATPase Activity
• The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
• 375 Studies on Antifungal Antibiotics from Ellisiodothis inquinans L1588-A8
• Leptosphaeria sp.の生産する抗真菌剤 : 有機化学・天然物化学
• Design and Test of a Lens and Reflector System of an Electrostatic Ion Trap for Molecular Physics
• 「北海道産の超強力・強力小麦粉を用いた新高付加価値食品の開発」コンソーシアム
• 乳酸菌のH^+-ATPase欠損変異株に関する研究 : 微生物
• 藪田基金補助による研究小集会報告 : Horace G. Cutler, Gary Strobel両博士講演会
• FE-P10 Property improvement of rice by the addition of enzymes that are active during cooking(Section IX Food Engineering)
• FE-P9 Mechanism of deamidation of food proteins by cation-exchange resins of the carboxylate type(Section IX Food Engineering)
• Enhanced Calcium Absorption in the Small Intestine by a Phytate-removed Deamidated Soybean Globulin Preparation
• Influence of Phytate Removal and Structural Modification on the Calcium-binding Properties of Soybean Globulins
• Analysis of Water Sorption Behavior of Native and Denatured Proteins by Solution Thermodynamics
• Analysis of Volatile Components in Essential Oil of Upland Wasabi and Their Inhibitory Effects on Platelet Aggregation
• 酵素反応によってイヌリンより生産されたジフルクトース-アンヒドリドIIIの物理的, 化学的および生理学的性質
• 411 味噌モロミ由来の耐塩性酵母の分類学的多様性(分類・系統・遺伝学,一般講演)
• 味噌もろみ中のZygosaccharomyces rouxii M2株の菌数迅速モニタリング法の開発
• 1053 嫌気性ジャーファーメンターを用いた腸内細菌の培養条件の検討
• Absidia corymbiferaのα-ガラクトシダーゼ遺伝子のクローニングおよび大腸菌での発現
• Pseudomonas azelaica YA-1が生産するニッケルプロトポルフィリン分解酵素の精製と性質
• 252 微生物による砂糖からのDFA IV生産 : その2 精製・物性
• 251 微生物による砂糖からのDFA IV生産 : その1 製造法
• 嫌気性ジャーファーメンターを用いた腸内細菌の培養条件とモニタリングの検討
• 発酵研究のフロンティア(第60回大会シンポジウム報告)
• Metabolism of DFA III by Arthrobacter sp. H65-7 : Purification and Properties of a DFA III Hydrolysis Enzyme (DFA IIIase)
• 634 Arthrobacter sp. H65-7株のDFA III分解酵素の精製と詣性質
• 3.乳酸菌のATPase欠損変異株の解析 : 酸性条件におけるATPase遺伝子の発現調節(乳酸菌の総合的産業利用に関する研究 : 平成9年度乳酸菌工学研究部会報告)
• 乳酸菌 Lactococcus lactis subsp. lactisのH^+-ATPase遺伝子のクローニング
• 乳酸菌のATPase欠損変異株の解析 : 酸性条件におけるATPase遺伝子の発現調節
• Purification and Characterization of D-Xylose Isomerase from Bifidobacterium adolescentis
• Industrial production of difructose anhydride III(DFA III) from crude inulin extracted from chicory roots using Arthrobacter sp. H65-7 fructosyltransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• 2K15-2 Corynebacterium glutamicumのH^+-ATPase活性低下変異によるバリン生産能の向上(代謝工学・メタボローム・生体情報工学,一般講演)
• Production of Worcestershire Sauce by a Trickle Bed Bioreactor Using Comb-Shaped Ceramics Carriers
• Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve
• Effects of Indigenous Starter Cultures on the Microbial and Physicochemical Characteristics of Urutan, a Balinese Fermented Sausage(BREWING AND FOOD TECHNOLOGY)
• Cholic acid accumulation and its diminution by short-chain fatty acids in bifidobacteria
• 403 Detection of novel thermotolerant species, Lactobacillus thermotolerans, in human and animal faeces by culture-independent molecular techniques
• 1112 Identification of Lactic Acid Bacteria Isolated from Urutan by Using Polymerase Chain Reaction-Amplified Variable Regions of 16S rDNA
• Enhanced Glutamic Acid Production by a H+-ATPase-Defective Mutant of Corynebacterium glutamicum
• 402 DFA3 (Difructose anhydride 3)摂取におけるヒト腸内細菌叢のモニタリング(分類・系統・遺伝学,一般講演)
• イヌリン - 新糖質資源としての展望
• Arthrobacter sp. H65-7株のInulin fructotransferase (DFA III生産型)遺伝子のクローニング : 酵素
• 世界の乳酸菌研究の最前線 : 第8回乳酸菌シンポジウム(オランダ)に出席して
• 腸内乳酸菌の胆汁酸取込み能とプロバイオティクス機能
• 大学院博士課程の危機的状況
• 腸内乳酸菌に対する胆汁酸の生育阻害機構のエネルギー代謝解析(メンブレン・ストレスバイオテクノロジー)
• 1H14-1 大腸菌 K-12 株の F_1-ATPase 欠損変異株の定量的プロテオーム解析
• 1H13-5 Corynebacterium glutamicum の H^+-ATPase 活性低下変異によるグルタミン酸生産能の向上
• Molecular Cloning of the Gene Encoding the di-D-Fructofuranose 1,2':2,3' Dianhydride Hydrolysis Enzyme (DFA IIlase) from Arthrobacter sp. H65-7
• Characterization of a Mutant of Lactococcus lactis with Reduced Membranebound ATPase Activity under Acidic Conditions
• Acid Sensitivity of a Mutant of Lactococcus lactis subsp. lactis C2 with Reduced Membrane-bound ATPase Activity
• Identification of a Putative Vacuolar Serine Protease Gene in the Rice Blast Fungus, Magnaporthe grisea(Biochemistry & Molecular Biology)
• Establishment of a New Cross of the Rice Blast Fungus Derived from Japanese Differential Strain Ina168 and Hermaphroditic Rice Pathogen Guy11(Microbiology & Fermentation Technology)
• Molecular Structure of rDNA Repeat Unit in Magnaporthe grisea
• L-Tryptophan Production by a Pyruvic Acid-Producing Escherichia coli Strain Carrying the Enterobacter aerogenes Tryptophanase Gene
• Enzymatic Synthesis of L-Tryptophan by Enterobacter aerogenes Tryptophanase Highly Expressed in Escherichia coli, and Some Properties of the Purified Enzyme
• Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry
• Molecular Cloning and Analysis of a Lipase Gene from Pseudomonas fluorescens No.33
• Pyruvic Acid Production by an F_1-ATPase-defective Mutant of Escherichia coli W1485lip2
• NP-101A, Antifungal Antibiotic from Streptomyces aurantiogriseus NPO-101
• Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
• Change of Proteins in Instant Chinese Noodle by the Fermentation of Lactobacillus plantarum NRIC 0380 Affects the Noodle Quality
• Identification and Characterization of a Karyotypic Mutation in Magnaporthe grisea
• Host Species-specific Repetitive DNA Sequence in the Genome of Magnaporthe grisea, the Rice Blast Fungus
• High Yield Methanol Formation in a CH_4-O_2-NO_2 Gaseous Selective Oxidation at 1 atm
• Production of Levanbiose by a Levan-degrading Enzyme from Streptomyces exfoliatus F3-2
• Induction and Catabolite Repression Mechanisms of Cellulase in Fungi
• A Novel Screen for the Detection of Chitin Acting Antifungal Compounds
• Bacterial Adjunct to Improve the Quality of Alkaline Noodles
• Microbial Demetallization of Crude Oil : Nickel Protoporphyrin Disodium as a Model Organo-Metallic Substrate
• Molecular Cloning and High-Level Expression in Escherichia coli of Fungal α-Galactosidase from Absidia corymbifera IFO 8084
• Effects of DFA IV in Rats : Calcium Absorption and Metabolism of DFA IV by Intestinal Microorganisms
• Molecular Cloning of Levan Fructotransferase Gene from Arthrobacter nicotinovorans GS-9 and Its Expression in Escherichia coli
• Purification of Levan Fructotransferase from Arthrobacter nicotinovorans GS-9 and Production of DFA IV from Levan by the Enzyme
• Molecular Cloning of an Inulin Fructotransferase (Depolymerizing) Gene from Arthrobacter sp. H65-7 and Its Expression in Escherichia coli
• Quantitative Analysis of Saponins in a Tea-Leaf Extract and Their Antihypercholesterolemic Activity
• Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
• Improvement of Digestibility, Reduction in Allergenicity, and Induction of Oral Tolerance of Wheat Gliadin by Deamidation
• Difructose Anhydrides : Their Mass-Production and Physiological Functions
• Technological Advances in Bifidobacterial Molecular Genetics : Application to Functional Genomics and Medical Treatments

もっと見る 閉じる

スポンサーリンク