rDNA ITS Sequence of Rhizopus oryzae : Its Application to Classification and Identification of Lactic Acid Producers(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. To date, there were no easy methods to classify strains according to their acid production. The sequences of the ribosomal RNA-encoding DNA (rDNA) internal transcribed spacer (ITS) region of 64 strains of R. oryzae were analyzed and found to conserve mutations correspond to acid production. We have devised a way to use these mutations for a novel method to identify lactic-acid-producing Rhizopus oryzae, by designing specific polymerase chain reaction (PCR) primers on them. Touch down PCR using these primers amplified the ITS DNA of lactic acid producers specifically. By this method, we could isolate lactic acid producing strains from Indonesian fermented foods.
- 社団法人日本農芸化学会の論文
- 2003-08-23
著者
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ODA Yuji
Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro
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Sone Teruo
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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Asano Kozo
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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Sone Teruo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone Teruo
Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci
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TOMITA FUSAO
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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ABE Ayumi
Laboratory of Applied Microbiology, the Graduate School of Agriculture, Hokkaido University
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SUJAYA I
Laboratory of Applied Microbiology, the Graduate School of Agriculture, Hokkaido University
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SAITO Katsuichi
Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region
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冨田 房男
北海道大学農学部
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Sujaya Inengah
Northern Advancement Center For Science And Technology
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Abe Ayumi
Department Of Bioscience And Chemistry Graduate School Of Agriculture Hokkaido University
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Sone T
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone Teruo
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Sujaya I-nengah
Department Of Bioscience And Chemistry Graduate School Of Agriculture Hokkaido University
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Asano Kozo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Asano Kozo
北海道大学農学部応用生命科学専攻
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Tomita Fusao
Laboratory Of Applied Microbiology
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Tomita Fusao
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Shiomi Takuya
Fancl Res. Inst. Fancl Corp.
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Saito Katsuichi
Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region
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Saito Katsuichi
Memuro Upland Farming Research Station National Agricultural Research Center For Hokkaido Region
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Saito K
Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region
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Abe Ayumi
Research Faculty Of Agriculture Hokkaido University
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Oda Y
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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Oda Yuji
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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Asano Kozo
Laboratory Of Applied Microbiology Graduate Faculty Of Agriculture Hokkaido University
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Abe Ayumi
Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University
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