Enhancement of Glucose Metabolism a Pyruvic Acid-Hyperproducing Escherichia coli Mutant Defective in F_1-ATPase Activity

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概要

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• An F_1-ATPase-defective mutant of Escherichia coli K-12,strain TBLA-1,showing an enhanced pyruvic acid productivity compared with its parental strain W1485lip2,was characterized physiologically to elucidate the mechanisms of the enhancement in productivity. Cell of strain TBLA-1 producing pyruvic acid showed a higher rate of the oxygen consumption and a higher b-type cytochromes content than cells of strain W1485lip2.The activities of the phosphostransferase system for glucose uptake measured in terms of the level of phosphoenolpyruvate-dependent phosphorylation of methyl α-D-glucopyranoside and 2-deoxy-D-glucose using toluenized cells were higher in strain TBLA-1 than in strain W1485lip2. Among the activities of all the glycolytic enzymes, those of phosphoglycerate kinase and pyruvate kinase-I were found to be higher in strain TBLA-1 than in strain W1485lip2 during the logarithmic growth phase. These characteristics of strain TBLA-1 are discussed in relation to its enhanced pyruvic acid productivity.

• 社団法人日本生物工学会の論文
• 1997-02-25

著者

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• HENMI MASARU

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• TAKAOKA NAOHISA

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• HAYASHI CHIHO

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• TAKEZAWA YUJI

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• FUKUMORI YOSHIHIRO

  Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• Fukumori Yoshihiro

  Department Of Biology Faculty Of Science Osaka University

• Yokota A

  Hokkaido Univ. Hokkaido Jpn

• Henmi Masaru

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Hayashi Chiho

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Terasawa Yukio

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Takaoka Naohisa

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Fukumori Y

  Shinshu Univ. Nagano Jpn

• Fukumori Yoshihiro

  Department Of Biology Faculty Of Science Kanazawa University

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