Production of Levanbiose by a Levan-degrading Enzyme from Streptomyces exfoliatus F3-2

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概要

• 論文の詳細を見る

• Levan-assimilating microorganisms obtained from soil samples were screened for levan-degrading enzyme production. Among them, the strain F3-2 was found to produce an extracellular levan-degrading enzyme that hydrolyzed levan to a disaccharide identified as levanbiose. The strain F3-2 was identified as Streptomyces exfoliatus F3-2. This levan-degrading enzyme was specifically induced by levan, and the activity was increased to 2.58 units/ml by improvement of the culture conditions. The optimum temperature and pH for the enzyme reaction were 60℃ and 5.5, respectively. The crude levan-degrading enzyme was stable within a pH range of 3.0 to 9.0 and at up to 50℃. Using this crude levan-degrading enzyme, 50 mg/ml of levan was converted to levanbiose in amounts of 42 mg/ml.

• 社団法人日本農芸化学会の論文
• 1993-05-23

著者

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• KOJIMA ICHIRO

  Central Technical Research Laboratory, Nippon Oil Company Ltd.,

• Nakagawa M

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• Kondo Kazuya

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• Nakagawa Mari

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• Kondo Kazuya

  School Of Engineering Hokkaido University

• Kojima I

  Mercian Corporation Central Research Laboratories

• Kojima Ichiro

  Central Technical Research Laboratory Nippon Oil Company Ltd.

• Kondo Kazuya

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

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