Purification of Levan Fructotransferase from Arthrobacter nicotinovorans GS-9 and Production of DFA IV from Levan by the Enzyme

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概要

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• A bacterial strain, GS-9, isolated from soil as a levan-degrading microorganism produced an extracellular enzyme that converted levan into DFA IV. This strain was identified as Arthrobacter nicotinovorans. The DFA IV-producing enzyme was specifically induced by levan. The enzyme was purified 60-fold from culture supernatant to give a single band on SDS-PAGE. The molecular weight of this enzyme was 52,000 by SDS-PAGE and a monomer by gel filtration. The enzyme gave DFA IV as a main product (>75%), and fructose, levanbiose, and two unidentified oligosaccharides as minor products, and was identified as a novel levan fructotransferase.

• 社団法人日本農芸化学会の論文
• 1997-10-23

著者

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• SAITO KATSUICHI

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• 横田 篤

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Yokota Atsushi

  Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• GOTO Hiroko

  Laboratory for Structure and Function, Tokai University School of Medicine

• Saito Katsuichi

  Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region

• Saito Katsuichi

  Memuro Upland Farming Research Station National Agricultural Research Center For Hokkaido Region

• Saito Katsuichi

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University

• Yokota A

  Hokkaido Univ. Hokkaido Jpn

• Goto Hiroko

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Goto Hiroko

  Laboratory For Structure And Function Tokai University School Of Medicine

• Saito K

  Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region

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