Isolation of a Cadmium-releasing Bacterium and Characterization of Its Novel Protease
スポンサーリンク
概要
- 論文の詳細を見る
Microorganisms were screened for their ability to release cadmium from scallop hepatopancreas, which is the main residue after removing of the edible parts of scallop. The isolated strain, 23-0-11, identified as Arthrobacter nicotinovorans, secreted a protease which released cadmium from scallop hepatopancreas into the liquid medium. The molecular mass of the enzyme was estimated to be 27 kDa. The sequence of the 15 N-terminal amino acids of the protease showed no close similarity with any other protein. Compared with a commercial enzyme, the purified protease had greater ability to release cadmium. The enzyme activity was greatest at 50 °C and pH 7.0, and was enhanced in the presence of Ca2+, Mg2+ and Mn2+, while being strongly inhibited by Co2+. The inhibition profile by the serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF), confirmed that the protease belonged to the serine protease family.
- 社団法人 日本農芸化学会の論文
- 2004-08-23
著者
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Yokota Atsushi
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
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Asano Kozo
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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TOMITA FUSAO
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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冨田 房男
北海道大学農学部
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横田 篤
Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University
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REN Shukun
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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Yokota Atsushi
Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo
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横田 篤
北大 大学院農学研究院
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Yokota Atsushi
Laboratory Of Bioorganic Chemistry
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Yokota A
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Yokota Atsushi
Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University
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Yokota Atsushi
Hokkaido Univ. Sapporo Jpn
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Yokota Atsushi
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Asano Kozo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Asano Kozo
北海道大学農学部応用生命科学専攻
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Tomita Fusao
Laboratory Of Applied Microbiology
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Tomita Fusao
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Tomita Fusao
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Yokota Atsushi
Lab. Microb. Physiol. Grad. Sch. Agric. Hokkaido Univ.
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Yokota A
Hokkaido Univ. Hokkaido Jpn
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Ren S
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Ren Shukun
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Asano Kozo
Laboratory Of Applied Microbiology Graduate Faculty Of Agriculture Hokkaido University
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