Molecular Cloning and Analysis of a Lipase Gene from Pseudomonas fluorescens No.33
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概要
- 論文の詳細を見る
The gene encoding an extracellular lipase from Pseudomonas fluorescens No. 33 was cloned and sequenced. A single open reading frame consisting of 1,428 nucleotides that encoded a mature protein of 476 amino acids was recognized. Sequence analysis showed that the deduced molecular weight of 50,209 agreed with the molecular weight of the purified lipase as measured by SDS-PAGE and the lipase lacked a signal peptide. The presence of a repeating motif, GXXGXDXXX, suggested that the lipase might be exported and secreted via a system that involves the ATP-binding cassette protein.
- 社団法人日本農芸化学会の論文
- 1998-11-23
著者
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TOMITA FUSAO
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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KUMURA Haruto
Laboratory of Dairy Science, Research Group of Animal Product Science, Division of Bioresources and
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SHIMAZAKI Kei-ichi
Laboratory of Dairy Science, Research Group of Animal Product Science, Division of Bioresources and
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Kumura H
Hokkaido Univ. Hokkaido Jpn
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Kumura Haruto
北海道大学 農学研究科畜産科学科
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Kumura Haruto
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
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Kumura Haruto
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
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Tomita Fusao
Laboratory Of Applied Microbiology
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Shimazaki K
Hokkaido Univ. Hokkaido Jpn
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Shimazaki Kei-ichi
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
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SAKURAI Hiroaki
Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,
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HIROSE Shuji
Laboratory of Dairy Science, Division of Bioresources and Product Science
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MIKAWA Katsuhiko
Laboratory of Dairy Science, Division of Bioresources and Product Science
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Hirose Shuji
Laboratory Of Dairy Science Division Of Bioresources And Product Science
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Mikawa Katsuhiko
Laboratory Of Dairy Science Division Of Bioresources And Product Science
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Tomita Fusao
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Sakurai Hiroaki
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Shimazaki K
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
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SHIMAZAKI Kei-ichi
Laboratory of Dairy Chemistry, Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine
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