Primary Culture of Porcine Mammary Epithelial Cells as a Model System for Evaluation of Milk Protein Expression(Biochemistry & Molecular Biology)

概要

論文の詳細を見る
Porcine mammary epithelial cells were isolated to culture on collagen gel followed by gel floating treatment to evaluate differentiation under the culture conditions of serum-free medium, supplemented with combinations of insulin, hydrocortisone, and prolactin. After the culture period, the mammary cells attached to the collagen gels were recovered to observe expression of β-casein, β-lactoglobulin, and lactoferrin by reverse transcriptase polymeric chain reaction method. Expression of β-casein was observed in the presence of insulin, hydrocortisone, and prolactin whereas transcription of β-lactoglobulin and lactoferrin occured irrespective of hydrocortisone and prolactin. Immunoblot analysis demonstrated synthesis and secretion of lactoferrin in the fraction of recovered cells and the culture medium.
社団法人日本農芸化学会の論文
2001-09-23