Expression and Characterization of Bovine Lactoperoxidase by Recombinant Baculovirus(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Lactoperoxidase (LPO) is a heme-containing oxidation-reduction enzyme present in milk. In this study, the gene encoding bovine lactoperoxidase (bLPO) was inserted into a baculovirus transfer vector, and a recombinant virus expressing bLPO was isolated. A bLPO-related recombinant baculovirus-expressed protein of 78 kDa was detected using anti-bLPO antibodies. After digestion with N-glycosidase F, the molecular weight of the recombinant bLPO (rbLPO) decreased. In addition, rbLPO reacted with lectin, indicating that the protein was glycosylated. The rbLPO activity and heme content in the culture supernatants increased upon addition of δ-aminolevulinic acid, which is a heme precursor. Differences in the δ-aminolevulinic acid-dependent circular dichroism spectrum and rbLPO pepsin hydrolysis were observed. These results suggest that the secondary structure and structural stability of rbLPO depends on the heme environment. Our data suggest that this bLPO expression system is useful for studying structure, catalytic mechanisms, and biological function.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
-
Kumura H
Hokkaido Univ. Hokkaido Jpn
-
Kumura Haruto
北海道大学 農学研究科畜産科学科
-
Kumura Haruto
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
-
Kumura Haruto
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
-
Shimazaki K
Hokkaido Univ. Hokkaido Jpn
-
Shimazaki Kei-ichi
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
-
Shimazaki Kei-ichi
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
-
Tanaka Tetsuya
Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokk
-
SATO Sanae
Dairy Science Laboratory, Graduate School of Agriculture, Hokkaido University
-
Sato Sanae
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
-
Shimazaki Kei-ichi
Dairy Chemistry Research Laboratory Department Of Bioindustrial Chemistry Division Of Bioresource Ch
-
Sato S
Hokkaido Univ. Hokkaido Jpn
-
Tanaka T
Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University
-
Shimazaki K
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
-
SHIMAZAKI Kei-ichi
Dairy Chemistry Laboratory, Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine
関連論文
- 精密ろ過膜とオンライン乳酸コントローラーを備えた培養システムを用いたPropionibacterium shermaniiによるビフィズス菌特異的増殖促進物質(BGS)の生産(生物工学論文賞)
- Growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria(BREWING AND FOOD TECHNOLOGY)
- Toxoplasma gondiiにおける牛ラクトフェリン結合タンパク質の検出(寄生虫病)
- バイオマスプラスチック製濾過膜の開発と応用 (特集 新しいバイオマス利活用技術)
- 1Mp22 米タンパク質由来ペプチドのヒト病原菌に対する抗菌作用とその機構の解明(食品科学・食品工学,一般講演)
- 1Mp21 酒造工程における乳酸菌由来バクテリオシンの火落菌に対する殺菌作用(食品科学・食品工学,一般講演)
- 非対称型デプスフィルターによる非圧縮性粒子懸濁液の精密濾過
- 食品微生物を用いた有用生理活性物質の生産
- 乳酸菌バクテリオシンを用いた清酒の火落ち防止技術の開発
- ポリ乳酸の多孔質化・複合化による機能性材料の開発(循環型社会を支えるラクテートインダストリーの新たな研究潮流)
- Casein Digestion by Debaryomyces hansenii Isolated from Cheese(Food & Nutrition Science)
- Sequence analysis of porcine polymeric immunoglobulin receptor from mammary epithelial cells present in colostrum
- Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for the enzymatic hydrolysis of rice straw(ENVIRONMENTAL BIOTECHNOLOGY)
- Production of Bifidogenic Growth Stimulator by Co-cultivation of Propionibacterium shermanii and Lactobacillus Strain Using Lactose as a Carbon Source
- 3P-1167 Porphyromonas gingivalisに対して抗菌活性を有する新規米由来タンパク質の検索と同定(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- 3P-1166 米タンパク質由来CHペプチドの口腔細菌に対する抗菌作用とその機構の解明(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- Inhibitory Effect of Juniperonic Acid (Δ-5c,11c,14c,17c-20:4,ω-3) on Bombesin-Induced Proliferation of Swiss 3T3 Cells(Biochemistry)
- Growth promotion and cell binding ability of bovine lactoferrin to Bifidobacterium longum
- Bovine lactoferrin region responsible for binding to bifidobacterial cell surface proteins
- Visualization of Bovine Lactoferrin Binding to Bifidobacteria
- Comparison of Growth Promoting Effects on Bifidobacterium spp. by Bovine Lactoferrin Hydrolysates
- FE-P6 Growth-inhibitory activity of antimicrobial peptides from rice against oral pathogenic bacteria and clarification of their action mechanism(Section IX Food Engineering)
- Production of 1, 2-Didocosahexaenoyl Phosphatidylcholine by Bonito Muscle Lysophosphatidylcholine/Transacylase
- Lipoxygenase-1 from Soybean Seed Inhibiting the Activity of Pancreatic Lipase
- Properties of Phospholipase A_1/Transacylase in the White Muscle of Bonito Euthynnus pelamis(Linnaeus)
- Susceptibility of bovine osteopontin to chymosin
- Susceptibilities against Bovine Lactoferrin with Microorganisms Isolated from Mastitic Milk
- Expression and Characterization of Bovine Lactoperoxidase by Recombinant Baculovirus(Biochemistry & Molecular Biology)
- Primary Culture of Porcine Mammary Epithelial Cells as a Model System for Evaluation of Milk Protein Expression(Biochemistry & Molecular Biology)
- Molecular Cloning and Analysis of a Lipase Gene from Pseudomonas fluorescens No.33
- Monoclonal Antibody against Bovine Lactoferricin® and Its Epitopic Site
- Structural and Immunochemical Studies of Bovine Anitmicrobial Peptide 'lactoferricin'
- Expression of Human Lactoferricin in HC11 Cells
- Evaluation of Pretreatment with Pleurotus ostreatus for Enzymatic Hydrolysis of Rice Straw(ENVIRONMENTAL BIOTECHNOLOGY)
- Production of L-Lactic Acid by Simultaneous Saccharification and Fermentation Using Unsterilized Defatted Rice Bran as a Carbon Source and Nutrient Components
- Production of a Mixture of Antimicrobial Organic Acids from Lactose by Co-Culture of Bifidobacterium longum and Propionibacterium freudenreichii
- Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria
- Inhibition of Lymphocyte Kinase Lck and Phosphatidylinositol 3-kinase by a Novel Immunosuppressant, Lymphostin(Biochemistry & Molecular Biology)
- Characterization of Extracellular RNAs Produced by the Marine Photosynthetic Bacterium Rhodovulum sulfidophilum
- Milk and Milk Products in Bangladesh
- Synthesis and Herbicidal Activity of Pyridinyloxyphenoxypropionamides
- Determination of the (2E, 4E)-Geometry of the 3,5-Dimethyl-2,4-diene System of Antibiotic 1233A by NMR Comparison with the Four Geometrical Isomers of Methyl 3,5-Dimethyl-2,4-heptadienoate
- Culture Conditions for Improvement of L-Threonine Production Using a Genetically Self-cloned L-Threonine Hyperproducing Strain of Escherichia coli K-12
- Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw
- Spawning Habitat and Nest Depth of Female Dolly Varden Salvelinus malma of Different Body Size
- Escherichia coli tRNAs Are Resistant to the Hyperprocessing Reaction of Homologous E. coli Ribonuclease P Ribozyme(Biochemistry & Molecular Biology)
- Kinetics of Hyperprocessing Reaction of Human Tyrosine tRNA by Ribonuclease P Ribozyme from Escherichia coli(Biochemistry & Molecular Biology)
- Another Cut for Lysine tRNA:Application of the Hyperprocessing Reaction Reveals Another Stabilization Strategy in Metazoan Lysine tRNAs^1
- Human Tyrosine tRNA Is Also Internally Cleavable by E. coli Ribonuclease P RNA Ribozyme in Vitro(Biochemistry & Molecular Biology)
- Application of a Metal Switch to Aqualysin I, a Subtilisin-type Bacterial Serine Protease, to the S3 Site Residues, Ser102 and Gly131
- Identification and Designing of the S3 Site of Aqualysin I, a Thermophilic Subtilisin-Related Serine Protease
- Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO
- Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1 : Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg
- P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor
- Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1
- Polymerization Degree of Oligomethionine to Determine Its Bioavailability When Added to a Low-protein Diets
- Correlation between Molecular Weight Distribution of Oligo-L-methionine Prepared by Papain-catalyzed Polymerization and Its Supplementary Effect in a Low Protein Diet
- New Selective Grass Herbicides and Their Hydrolytic Properties as Pro-herbicides
- The Natural Intron Sequence of Human Tyrosine Pre-transfer RNA Is Not a Temporal Stabilizer for Cloverleaf Structure
- Examining the Bases of the J3/4 Domain of Escherichia coli Ribonuclease P
- The Protein Component of Bacterial Ribonuclease P Flickers the Metal Ion Response to the Substrate Shape Preference of the Ribozyme(Biochemistry & Molecular Biology)
- Comparative Analyses of Hairpin Substrate Recognition by Escherichia coli and Bacillus subtilis Ribonuclease P Ribozymes(Biochemistry & Molecular Biology)
- Substrate Shape Specificity of E. coli RNase P Ribozyme Is Dependent on the Concentration of Magnesium Ion
- Formation of Lysophosphatidic Acid, a Wound-Healing Lipid, during Digestion of Cabbage Leaves
- Extracellular tRNAs of the Marine Photosynthetic Bacterium Rhodovulum sulfidophilum Are Not Aminoacylated
- 非対称型デプスフィルターによる細菌懸濁液の精密濾過
- Expression of Bovine Lactoferrin C-lobe in Rhodococcus erythropolis and Its Purification and Characterization
- Mutational Analysis of the Length of the J3/4 Domain of Escherichia coli Ribonuclease P Ribozyme
- Susceptibility of Bovine Lactoferrin to Plasmin and Chymosin(Biological Chemistry)
- Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying
- Artificial RNA aptamer production by the marine bacterium Rhodovulum sulfidophilum : Improvement of the aptamer yield using a mutated transcriptional promoter(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Synergistic fungicidal activities of polymyxin B and ionophores, and their dependence on direct disruptive action of polymyxin B on fungal vacuole
- Antimycin A-induced cell death depends on AIF translocation through NO production and PARP activation and is not involved in ROS generation, cytochrome c release and caspase-3 activation in HL-60 cells
- Enhancement effect of N-methyl-N"-dodecylguanidine on the vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans
- The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin
- Dependence of vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae
- Protective Effect of Lactoferricin against Toxoplasma gondii Infection in Mice.
- Kinetic Analysis of Reactivity of Amino Groups in Bovine Secretory IgA with 2, 4, 6-Trinitrobenzenesulfonic Acid