Application of a Metal Switch to Aqualysin I, a Subtilisin-type Bacterial Serine Protease, to the S3 Site Residues, Ser102 and Gly131

スポンサーリンク

概要

• 論文の詳細を見る

• We applied 'metal switch'experiments to the S3 site residues, Ser102 and Gly131,of aqualysin I, a subtilisintype serine protease. We showed that two histidines introduced at these positions did take part in histidine-metal-histidine bridge formation, and metal ions inhibited the protease activities. These results indicate that two histidines are near each other, and both side chains are metal-accessible. This is the first report on application of the metal-switch technique to a subtilisin-related enzyme.

• 社団法人日本農芸化学会の論文
• 2000-09-23

著者

• Ohta T

  Hokkaido Food Processing Res. Center Hokkaido Jpn

• Tanaka T

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka T

  Hiroshima Univ. Higashi Hiroshima Jpn

• Mori Hiroaki

  Department Of Agricultural Chemistry The University Of Tokyo

• Tanaka T

  Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center

• Tanaka Takaaki

  Graduate School Of Science And Technology Niigata University

• Kikuchi Yo

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• Kikuchi Yo

  Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O

• TANAKA Terumichi

  Division of Life Science and Biotechnology, Department of Ecological Engineering, Toyohashi Universi

• Matsuzawa H

  Department Of Bioscience And Biotechnology Aomori University

• MATSUZAWA Hiroshi

  Aomori University

• OHTA Takahisa

  Kogakuin University

• Tanaka T

  Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University

• Tanaka Terumichi

  Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O

• Kikuchi Yo

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• Tanaka Takaaki

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Tanaka Terumichi

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

関連論文

• 精密ろ過膜とオンライン乳酸コントローラーを備えた培養システムを用いたPropionibacterium shermaniiによるビフィズス菌特異的増殖促進物質(BGS)の生産(生物工学論文賞)
• Growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria(BREWING AND FOOD TECHNOLOGY)
• バイオマスプラスチック製濾過膜の開発と応用 (特集 新しいバイオマス利活用技術)
• 1Mp22 米タンパク質由来ペプチドのヒト病原菌に対する抗菌作用とその機構の解明(食品科学・食品工学,一般講演)
• 1Mp21 酒造工程における乳酸菌由来バクテリオシンの火落菌に対する殺菌作用(食品科学・食品工学,一般講演)
• 非対称型デプスフィルターによる非圧縮性粒子懸濁液の精密濾過
• 食品微生物を用いた有用生理活性物質の生産
• 乳酸菌バクテリオシンを用いた清酒の火落ち防止技術の開発
• ポリ乳酸の多孔質化・複合化による機能性材料の開発(循環型社会を支えるラクテートインダストリーの新たな研究潮流)
• 1Gp07 ヒラタケによって脱リグニン処理した稲わらを用いたエタノールの生産(培養工学,一般講演)
• 1Gp08 酵素を用いた多収米の液化糖化の最適化とエタノール生産の低コスト化(培養工学,一般講演)
• 2Ip07 清酒製造工程における乳酸菌バクテリオシンの火落菌に対する増殖阻害作用(食品科学・食品工学,一般講演)
• 2Ip08 歯周病菌に対する米由来抗菌タンパク質の検索および分離同定(食品科学・食品工学,一般講演)
• 2Ip09 抗菌活性測定法の高感度化とそれを用いた植物由来抗菌タンパク質の検索(食品科学・食品工学,一般講演)
• Formation of Lysophosphatidic Acid, a Wound-Healing Lipid, during Digestion of Cabbage Leaves
• Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for the enzymatic hydrolysis of rice straw(ENVIRONMENTAL BIOTECHNOLOGY)
• Production of Bifidogenic Growth Stimulator by Co-cultivation of Propionibacterium shermanii and Lactobacillus Strain Using Lactose as a Carbon Source
• Production of Extracellular Bifidogenic Growth Stimulator(BGS) from Propionibacterium shermanii Using a Bioreactor System with a Microfiltration Module and an On-line Controller for Lactic Acid Concentration(BIOCHEMICAL ENGINEERING)
• Production of Extracellular Bifidogenic Growth Stimulator by Anaerobic and Aerobic Cultivations of Several Propionibacterial Strains(BIOCHEMICAL ENGINEERING)
• 3P-1167 Porphyromonas gingivalisに対して抗菌活性を有する新規米由来タンパク質の検索と同定(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
• 3P-1166 米タンパク質由来CHペプチドの口腔細菌に対する抗菌作用とその機構の解明(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
• Photo-control of Aggregate Behavior of Coiled-coil Motif Peptides Using Azobenzene Derivatives
• RP-1551s, a Family of Azaphilones Produced by Penicillium sp., Inhibit the Binding of PDGF to the Extracellular Domain of Its Receptor
• EI-1507-1 and-2, Novel Interleukin-1βConverting Enzyme Inhibitors Produced by Streptomyces sp. E-1507
• RES-1214-1 and-2, Novel Non-peptidic Endothelin Type A Receptor Antagonists Produced by Pestalotiopsis sp.
• Inhibitory Effect of Juniperonic Acid (Δ-5c,11c,14c,17c-20:4,ω-3) on Bombesin-Induced Proliferation of Swiss 3T3 Cells(Biochemistry)
• Conformationally Stabilized Antagonists for the Clearance Receptor of Atrial Natriuretic Peptide with Enhanced In Vitro and In Vivo Biological Activity
• Hybrid-Type Peptides Constructed from RES-701-1, an ET_B-Selective Antagonist Peptide, and ET-1
• Amino Acid Residues in the Allosteric Site of L-Lactate Dehydrogenase from Bifidobacterium longum(Biological Chemistry)
• APOPTOTIC SIGNAL AT THE FINAL STAGE OF GASTRULATION IN SEA URCHIN EMBRYO(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• CHANGES IN THE CELL NUMBER OF SEA URCHIN EMBRYOS AND DETECTION OF APOPTOTIC CELLS BY COMETASSAY(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• FE-P6 Growth-inhibitory activity of antimicrobial peptides from rice against oral pathogenic bacteria and clarification of their action mechanism(Section IX Food Engineering)
• Production of 1, 2-Didocosahexaenoyl Phosphatidylcholine by Bonito Muscle Lysophosphatidylcholine/Transacylase
• Lipoxygenase-1 from Soybean Seed Inhibiting the Activity of Pancreatic Lipase
• Properties of Phospholipase A_1/Transacylase in the White Muscle of Bonito Euthynnus pelamis(Linnaeus)
• DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
• DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
• Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
• Stimulatory Effect of Human Lactoferrin on DNA Synthesis in BALB/c 3T3 Cells
• Evaluation of Pretreatment with Pleurotus ostreatus for Enzymatic Hydrolysis of Rice Straw(ENVIRONMENTAL BIOTECHNOLOGY)
• Production of L-Lactic Acid by Simultaneous Saccharification and Fermentation Using Unsterilized Defatted Rice Bran as a Carbon Source and Nutrient Components
• Production of a Mixture of Antimicrobial Organic Acids from Lactose by Co-Culture of Bifidobacterium longum and Propionibacterium freudenreichii
• EI-1511-3, -5 and EI-1625-2, Novel Interleukin-1β Converting Enzyme Inhibitors Produced by Streptomyces sp. E-1511 and E-1625 III. Biochemical Properties of EI-1511-3, -5 and EI-1625-2
• EI-1511-3, -5 and EI-1625-2, Novel Interleukin-1β Converting Enzyme Inhibitors Produced by Streptomyces sp. E-1511 and E-1625 I. Taxonomy of Producing Strain, Fermentation and Isolation
• RES-1149-1 and -2, Novel Non-peptidic Endothelin Type B Receptor Antagonists Produced by Aspergillus sp. III. Biochemical Properties of RES-1149-1, -2 and Structure-activity Relationships
• RES-1149-1 and -2, Novel Non-peptidic Endothelin Type B Receptor Antagonists Produced by Aspergillus sp. I. Taxonomy of Producing Strain, Fermentation, Isolation, and Physico-chemical and Biological Properties
• RES-701-2,-3 and-4, Novel and Selective Endothelin Type B Receptor Antagonists Produced by Streptomyces sp.I.Taxonomy of Producing Strains, Fermentation, Isolation, and Biochemical Properties
• Transformation from Geraniol, Nerol and Their Glucosides into Linalool and α-Terpineol during Shochu Distillation
• Identification of Monoterpene Alcohol β-Glucosides in Sweet Potatoes and Purification of a Shiro-koji β-Glucosidase(Food & Nutrition)
• Regulation of Protein Expression using Designed α-Helical Coiled-coil in E. coli
• Carboxypeptidase Taq, a Thermostable Zinc Enzyme, from Thermus aquaticus YT-1 : Molecular Cloning, Sequencing, and Expression of the Encoding Gene in Escherichia coli
• Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
• Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria
• Antioxidant Activity of Ferulic Acid β-Glucuronide in the LDL Oxidation System
• Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
• Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
• Tryptophan-Niacin Metabolism in Liver Cirrhosis Rat Caused by Carbon Tetrachloride
• Effects of Various Kinds of Dietary Amino Acids on the Hepatotoxic Action of D-Galactosamine in Rats
• Effect of Indigestible Oligosaccharides on the Hepatotoxic Action of D-Galactosamine in Rats
• Change of Tryptophan-Niacin Metabolism in D-Galactosamine-Induced Liver Injury in Rat
• Effect of Dietary L-Glutamine on the Hepatotoxic Action of D-Galactosamine in Rats
• Effects of Dietary Gluten on the Hepatotoxic Action of Galactosamine and/or Endotoxin in Rats
• Suppression of Rat Hepatic α-Amino-β-carboxymuconate-ε-semialdehyde Decarboxylase (ACMSD) Activity by Linoleic Acid in Relation to Its Induction by Glucocorticoids and Dietary Protein
• Hyperexpression and Analysis of cho B Encoding Cholesterol Oxidase of Brevibacterium sterolicum in Escherichia coli and Streptomyces lividans
• PDE4 inhibitor (GPD-1116) prevents senescence-accelerated mice P1 strain (SAMP1) from developing cigarette smoke-induced emphysema.(Annual Report from the Division of Biomedical Research Resources, Juntendo University Graduate School of Medicine)
• Low Temperature Sensitization in the Weld Metal of Type 308 Stainless Steel and Its Improvement by Laser Surface Melting Treatment
• Synthesis and Herbicidal Activity of Pyridinyloxyphenoxypropionamides
• Reddish Escherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporphyrinogen III Methylase : Cloning, Sequencing, and Expression of the Gene
• Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and Comparison of the Properties of the Enzymes Produced in Escherichia coli
• A Neopullulanase-type α-Amylase Gene from Thermoactinomyces vulgaris R-47
• Culture Conditions for Improvement of L-Threonine Production Using a Genetically Self-cloned L-Threonine Hyperproducing Strain of Escherichia coli K-12
• Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
• Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
• Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
• Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw
• Application of a Metal Switch to Aqualysin I, a Subtilisin-type Bacterial Serine Protease, to the S3 Site Residues, Ser102 and Gly131
• Identification and Designing of the S3 Site of Aqualysin I, a Thermophilic Subtilisin-Related Serine Protease
• Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO
• Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1 : Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg
• P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor
• Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1
• Polymerization Degree of Oligomethionine to Determine Its Bioavailability When Added to a Low-protein Diets
• Correlation between Molecular Weight Distribution of Oligo-L-methionine Prepared by Papain-catalyzed Polymerization and Its Supplementary Effect in a Low Protein Diet
• New Selective Grass Herbicides and Their Hydrolytic Properties as Pro-herbicides
• Involvement of NH_2-Terminal Pro-sequence in the Production of Active Aqualysin I (a Thermophilic Serine Protease) in Escherichia coli(Biological Chemistry)
• Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
• Involvement of Glu-264 and Arg-235 in the Essential Interaction between the Catalytic Imidazole and Substrate for the D-Lactate Dehydrogenase Catalysis
• Expression of Aqualysin I (a Thermophilic Protease) in Soluble Form in Escherichia coli under a Bacteriophage T7 Promoter
• Production of Thermophilic Protease by Clucose-Controlled Fed-Batch Culture of Recombinant Escherichia coli
• Formation of Lysophosphatidic Acid, a Wound-Healing Lipid, during Digestion of Cabbage Leaves
• 非対称型デプスフィルターによる細菌懸濁液の精密濾過
• Poly(L-Lactic Acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying
• Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
• Secretion of Human Interleukin-2 in Biologically Active Form by Bacillus brevis Directly into Cultute Medium
• Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying
• Synergistic fungicidal activities of polymyxin B and ionophores, and their dependence on direct disruptive action of polymyxin B on fungal vacuole
• Antimycin A-induced cell death depends on AIF translocation through NO production and PARP activation and is not involved in ROS generation, cytochrome c release and caspase-3 activation in HL-60 cells
• Enhancement effect of N-methyl-N"-dodecylguanidine on the vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans
• The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin
• Dependence of vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae

もっと見る 閉じる

スポンサーリンク