Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
スポンサーリンク
概要
- 論文の詳細を見る
Functional role of Asn219 of aqualysin I, a thermostable serine protease from Thermus aquaticus, was investigated by using site-directed mutagenesis. Replacement of Asn219 with serine increased the catalytic efficiency (k_<cat>/K_m) for synthetic peptide substrates about twice as much as that of the wild type, while threonine replacement caused a slight decrease in the efficiency. Such replacements resulted in a significant change of k_<cat> rather than K_m, indicriting that the side chain in the vicinity of the catalytic residue Ser222 affects the catalytic rate constant.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
-
Kim Dong-wook
韓国
-
WAKAGI Takayoshi
Department of Biotechnology, The University of Tokyo
-
Wakagi T
Univ. Tokyo Tokyo Jpn
-
Kim D‐w
Catholic Univ. Korea Seoul Kor
-
KIM Dong-Wook
Department of Life Science, College of Natural Sciences, Chung-Ang University
-
Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
-
Kim Dong-wook
Department Of Hematology Catholic Blood And Marrow Transplantation Center College Of Medicine Seoul
-
Kim Dong‐wook
Dept. Of Electronic Eng. Kwang-woon University
-
MATSUZAWA Hiroshi
Department of Biotechnology, The University of Tokyo
-
Kim D‐w
Kwangyang Coll. Kwangyang Kor
-
LIN Shie-Jea
Department of Biotechnology, The University of Tokyo
-
RYUGO Yoko
Department of Biotechnology, The University of Tokyo
-
Ryugo Yoko
Department Of Biotechnology The University Of Tokyo
-
Lin Shie-jea
Department Of Biotechnology The University Of Tokyo
-
Kim Dong‐wook
Division Of Hematology Cml Clinical Research Institute 3rd Floor St Mary's Hospital The Catholi
-
Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
-
Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
-
Kim Dong-wook
Department Of Electrical Andel Ectronic Engineering Yonsei University
-
Matsuzawa Hiroshi
Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo
-
Kim Dong-wook
Molecular Genetics Research Institute The Catholic University Of Korea
-
Kim Dongho
Cancer Research Institute The Catholic University Of Korea
-
Kim Dong-Wook
Department of Applied Physics, Hanyang University, Ansan, Kyeonggi 426-791, Korea
-
Matsuzawa Hiroshi
Department of Bioscience and Biotechnology, Aomori University
-
MATSUZAWA Hiroshi
Department of Agricultural Chemistry, The University of Tokyo
関連論文
- A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
- Chronic myeloid leukemia in Asia
- Crystallographic and Mutational Analyses of Substrate Recognition of Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum
- Transglycosylation Activity of Dictyoglomus thermophilum Amylase A
- A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
- Purification, Characterization, and Steady-State Kinetics of a meta-Cleavage Compound Hydrolase from Pseudomonas fluorescens IP01
- The Possible Involvement of Copper-Containing Nitrite Reductase (NirK) and Flavohemoglobin in Denitrification by the Fungus Cylindrocarpon tonkinense
- Streptomyces griseus Enhances Denitrification by Ralstonia pickettii K50, Which Is Possibly Mediated by Histidine Produced during Co-Culture
- Characterization of a Novel Histone H3K36 Methyltransferase setd3 in Zebrafish
- 2-Oxoacid: Ferredoxin Oxidoreductase from the Thermoacidophilic Archaeon, Sulfolobus sp. Strain 7
- Risk-Adapted Preemptive Therapy for Cytomegalovirus Disease after Allogeneic Stem Cell Transplantation : A Single-Center Experience in Korea
- Risk-Adapted Preemptive Therapy for Cytomegalovirus Disease after Allogeneic Stem Cell Transplantation : A Single-Center Experience in Korea
- Hepatosplenic Tuberculosis Mimicking Disseminated Candidiasis in Patients With Acute Leukemia
- Necrotizing Enterocolitis : Experience of 27 Cases From a Single Korean Institution
- Comparison of 2 Preparative Regimens for Stem Cell Transplantation from HLA-Matched Sibling Donors in Patients with Advanced Myelodysplastic Syndrome
- Structural and Optical Properties of LiNbO_3 Filrns Grown by Pulsed Laser Deposition with a Shadow Mask
- Thermostable β-Galactosidase from an Extreme Thermophile, Thermus sp. A4 : Enzyme Purification and Characterization, and Gene Cloning and Sequencing
- Favorable outcomes of intravenous busulfan, fludarabine, and 400 cGy total body irradiation-based reduced-intensity conditioning allogeneic stem cell transplantation for acute myelogenous leukemia with old age and/or co-morbidities
- Cloning of the Gene for Inorganic Pyrophosphatase from a Thermoacidophilic Archaeon, Sulfolobus sp. Strain 7,and Overproduction of the Enzyme by Coexpression of tRNA for Arginine Rare Codon
- Low-Complexity Watermarking Based on Entropy Coding in H.264/AVC
- A New Coding Technique for Digital Holographic Video Using Multi-View Prediction(Advanced Image Technology)
- APOPTOTIC SIGNAL AT THE FINAL STAGE OF GASTRULATION IN SEA URCHIN EMBRYO(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- CHANGES IN THE CELL NUMBER OF SEA URCHIN EMBRYOS AND DETECTION OF APOPTOTIC CELLS BY COMETASSAY(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Spontaneous Pneumothorax Developed in Patients with Bronchiolitis Obliterans after Unrelated Hematopoietic Stem Cell Transplantation : Case Report and Review of the Literature
- DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
- DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
- Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
- Stimulatory Effect of Human Lactoferrin on DNA Synthesis in BALB/c 3T3 Cells
- Detection of the BCR-ABL Gene by Interphase Fluorescence In Situ Hybridization (iFISH) in Chronic Myelogenous Leukemia Patients after Hemopoietic Stem Cell Transplantation: The Feasibility of iFISH Monitoring of Therapeutic Response in Peripheral Blood
- Performance Improvement of OTD by Symbol Splitting
- A Real-Time Image Compressor Using 2-Dimensional DWT and Its FPGA Implementation(VLSI Design Technology and CAD)
- Carboxypeptidase Taq, a Thermostable Zinc Enzyme, from Thermus aquaticus YT-1 : Molecular Cloning, Sequencing, and Expression of the Encoding Gene in Escherichia coli
- Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
- Clinical profile of dasatinib in Asian and non-Asian patients with chronic myeloid leukemia
- Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
- Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
- PDE4 inhibitor (GPD-1116) prevents senescence-accelerated mice P1 strain (SAMP1) from developing cigarette smoke-induced emphysema.(Annual Report from the Division of Biomedical Research Resources, Juntendo University Graduate School of Medicine)
- Low Temperature Sensitization in the Weld Metal of Type 308 Stainless Steel and Its Improvement by Laser Surface Melting Treatment
- Reddish Escherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporphyrinogen III Methylase : Cloning, Sequencing, and Expression of the Gene
- Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and Comparison of the Properties of the Enzymes Produced in Escherichia coli
- Engineering Subtilisin E for Enhanced Stability and Activity in Polar Organic Solvents
- Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
- Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
- Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
- Role of Two α-L-Arabinofuranosidases in Arabinoxylan Degradation and Characteristics of the Encoding Genes from Shochu Koji Molds, Aspergillus kawachii and Aspergillus awamori(BREWING AND FOOD TECHNOLOGY)
- Purification and Characterization of a Feruloylesterase from Aspergillus awamori
- PE-429 Safety and Efficacy of Drug Eluting Stent in Patients with Acute Coronary Syndrome(PE072,Coronary Revascularization, PCI (DES) 7 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Society)
- Application of a Metal Switch to Aqualysin I, a Subtilisin-type Bacterial Serine Protease, to the S3 Site Residues, Ser102 and Gly131
- Identification and Designing of the S3 Site of Aqualysin I, a Thermophilic Subtilisin-Related Serine Protease
- Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO
- Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1 : Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg
- P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor
- Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1
- Content and Chemical Compositions of Cerebrosides in Lactose-assimilating Yeasts
- Effects of Growth Temperature on Cerebroside Content and Chemical Composition in Kluyveromyces lactis
- Involvement of NH_2-Terminal Pro-sequence in the Production of Active Aqualysin I (a Thermophilic Serine Protease) in Escherichia coli(Biological Chemistry)
- A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax
- Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
- Sodium-23 and Lanthanum-139 Nuclear Magnetic Resonance Studies of Cation Binding to Aqualysin I, a Thermostable Serine Protease(Analytical Chemistry)
- Involvement of Glu-264 and Arg-235 in the Essential Interaction between the Catalytic Imidazole and Substrate for the D-Lactate Dehydrogenase Catalysis
- Expression of Aqualysin I (a Thermophilic Protease) in Soluble Form in Escherichia coli under a Bacteriophage T7 Promoter
- Production of Thermophilic Protease by Clucose-Controlled Fed-Batch Culture of Recombinant Escherichia coli
- A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
- The Possible Involvement of Copper-Containing Nitrite Reductase (NirK) and Flavohemoglobin in Denitrification by the Fungus Cylindrocarpon tonkinense
- Streptomyces griseus Enhances Denitrification by Ralstonia pickettii K50, Which Is Possibly Mediated by Histidine Produced during Co-Culture
- Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats
- Morphology Control of Patterned Carbon Nanofiber Arrays for Field Emission Applications
- Characterization of a Novel Histone H3K36 Methyltransferase setd3 in Zebrafish
- Structural Properties and Resistance-Switching Behavior of Thermally Grown NiO Thin Films
- Reversible Resistance Switching Behaviors of Pt/NiO/Pt Structures
- Structural Explanation for the Acquisition of Glycosynthase Activity
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Conversion of Neopullulanase-α-Amylase from Thermoactinomyces vulgaris R-47 into an Amylopullulanase-Type Enzyme^1
- Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
- Mutational Analysis of Fungal Family 11 Xylanases on pH Optimum Determination
- Interactions between Glycoside Hydrolase Family 94 Cellobiose Phosphorylase and Glucosidase Inhibitors
- Long-term pattern of pleural effusion from chronic myeloid leukemia patients in second-line dasatinib therapy
- Crystal Structures of Glycoside Hydrolase Family 51 α-L-Arabinofuranosidase from Thermotoga maritima
- Survival of the Aerobic Denitrifier Pseudomonas stutzeri Strain TR2 during Co-Culture with Activated Sludge under Denitrifying Conditions
- Comprehensive therapeutic outcomes of frontline imatinib mesylate in newly diagnosed chronic phase chronic myeloid leukemia patients in Korea : feasibility assessment of current ELN recommendation
- Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Archaeal Aldehyde Dehydrogenase ST0064 from Sulfolobus tokodaii, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
- Allosteric and Kinetic Properties of L-Lactate Dehydrogenase from Thermus caldophilus GK24, an Extremely Thermophilic Bacterium
- Production of Thermophilic Extracellular Proteases (Aqualysins I and II) by Thermus aquaticus YT-1, an Extreme Thermophile
- Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor(ENVIRONMENTAL BIOTECHNOLOGY)
- Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2
- Impact of pre-transplant marrow blasts on survival of allogeneic stem cell transplantation in adult acute myeloid leukemia