Sodium-23 and Lanthanum-139 Nuclear Magnetic Resonance Studies of Cation Binding to Aqualysin I, a Thermostable Serine Protease(Analytical Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Aqualysin I has at least two Ca^<2+>-binding sites that have different affinities for Ca^<2+>. The binding of various metal ions to aqualysin I was studied using ^<23>Na- and ^<139>La-NMR spectrometry. Evidence is presented that Ca^<2+>, La^<3+>, and Na+ bind to the low-affinity Ca^<2+>-binding site of aqualysin I, but Mg^<2+> does not. Our results confirm that binding of metals at the low-affinity Ca^<2+>-binding site is essential for thermostabilization, since the addition of Mg^<2+> did not result in thermostabilization. La^<3+> was found to bind to both the low-affinity Ca^<2+>-binding site and an additional metal ion-binding site that can also be involved in the thermostabilization of aqualysin I.
- 社団法人日本農芸化学会の論文
- 2002-06-23
著者
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WAKAGI Takayoshi
Department of Biotechnology, The University of Tokyo
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Wakagi T
Univ. Tokyo Tokyo Jpn
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Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
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Yoshimura Etsuro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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MATSUZAWA Hiroshi
Department of Biotechnology, The University of Tokyo
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LIN Shie-Jea
Department of Biotechnology, The University of Tokyo
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Lin Shie-jea
Department Of Biotechnology The University Of Tokyo
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Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
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SUEFUJI Kyoko
Department of Biotechnology, The University of Tokyo
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Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Yoshimura Etsuro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suefuji Kyoko
Department Of Biotechnology The University Of Tokyo
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Matsuzawa Hiroshi
Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo
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Yoshimura Etsuro
Department of Agricultural Chemistry, The University of Tokyo
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Matsuzawa Hiroshi
Department of Bioscience and Biotechnology, Aomori University
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MATSUZAWA Hiroshi
Department of Agricultural Chemistry, The University of Tokyo
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