Reddish Escherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporphyrinogen III Methylase : Cloning, Sequencing, and Expression of the Gene
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概要
- 論文の詳細を見る
During shotgun cloning of an amylase gene, we found a transformant of Escherichia coli with a reddish color. The transformant produced highly water-soluble red pigments the molecular masses of which were less than 3000. The plasmid harbored by the transformant contained a DNA fragment derived from a strain of Bacillus stearothermophilus. Truncation of the insert DNA showed that an 1. 1-kbp Sau 3A-SalI fragment was responsible for the reddish colony. An open reading frame was found in the nucleotide sequence of the 1. 1-kbp DNA fragment. The production of the red pigment was accompanied by a colorless 28-kDa protein. The sequence of the 28-kDa protein was highly homologous to bacterial uroporphyrinogen III methylases participating in corrinoid biosynthesis. The 28-kDa protein was found to be a thermostable uroporphyrinogen III methylase.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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SAKAI Hiroshi
Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University
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Ohta T
Hokkaido Food Processing Res. Center Hokkaido Jpn
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Sakai Hideo
University Forest In Chichibu Graduate School Of Agricultural And Life Sciences The University Of To
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Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
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MATSUZAWA Hiroshi
Department of Biotechnology, The University of Tokyo
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Sakai H
Okayama Univ. Okayama Jpn
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MINOWA Takashi
Department of Agricultural Chemistry, The University of Tokyo
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OHTA Takahisa
Department of Agricultural Chemistry, The University of Tokyo
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Minowa T
Univ. Tokyo Tokyo Jpn
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SAKANO Yoshiyuki
Department of Applied Biological Science, Tokyo University of Agriculture Technology
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Ohta Takahisa
Department Of Agricultural Chemistry The University Of Tokyo
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Chou Pei-Lin
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Ohtsuka Mari
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Yamasat Kazuhide
Culture Collection Center of Tokyo University of Agriculture
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Ohtsuka Mari
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
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Chou Pei-lin
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Sakano Y
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Sakano Yoshiyuki
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Matsuzawa Hiroshi
Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo
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Sakai Hiroshi
Department Of Agricultural Chemistry The University Of Tokyo
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Matsuzawa Hiroshi
Department of Bioscience and Biotechnology, Aomori University
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SAKANO Yoshiyuki
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University
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MATSUZAWA Hiroshi
Department of Agricultural Chemistry, The University of Tokyo
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