Active Form of Dipteran-Specific Insecticidal Protein Cry11A Produced by Bacillus thuringiensis subsp. israelensis(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The nucleotide sequence of the cry11A gene from Bacillus thuringiensis subsp. israelensis strain HD522 was analyzed and the molecular characterization of Cry11A toxin was done. The 70-kDa Cry11A protoxin was processed in vitro into 36- and 32-kDa fragments by trypsin and into 34- and 32-kDa fragments by gut proteases from C. pipiens. These two processed fragments are associated together to form the heterodimer. The results of the binding assay with BBMV and the bioassay toward C. pipiens larvae suggested that the heterodimer was biologically as active as the non-digested Cry11A toxin and the intramolecular cleavage did not promote the insecticidal activity. These results suggested that a probable complex of the 36- or 34-kDa and 32-kDa fragments was also one of the possible active forms of Cry11A, and that the biological functions of Cry11A was not essentially affected by the intramolecular cleavage of the 70-kDa protein.
- 社団法人日本農芸化学会の論文
- 2002-03-23
著者
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SAKAI Hiroshi
Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Sen Kikuo
Department Of Bioscience And Biotechnology Shinshu University
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YAMAGIWA Masashi
Department of Bioscience and Biotechnology, Okayama University
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OGAWA Ruriko
Department of Bioscience and Biotechnology, Okayama University
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YASUDA Kohki
Department of Bioscience and Biotechnology, Okayama University
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NATSUYAMA Hisako
Department of Bioscience and Biotechnology, Okayama University
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Yamagiwa Masashi
Department Of Bioscience And Biotechnology Okayama University
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Ogawa Ruriko
Department Of Bioscience And Biotechnology Okayama University
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Yasuda Kohki
Department Of Bioscience And Biotechnology Okayama University
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Sakai Hiroshi
Department Of Agricultural Chemistry The University Of Tokyo
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Natsuyama Hisako
Department Of Bioscience And Biotechnology Okayama University
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Sen Kikuo
Department of Bioscience and Biotechnology Faculty of Agriculture, Shinshu University
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