Cloning and Nucleotide Sequence of the Pullulanase Gene of Thermus thermophilus HB8 and Production of the Enzyme in Escherichia coli(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
A 3.4-kb Sph1 fragment carrying the pullulanase gene of Thermus thermophilus HB8 was cloned. Based on the nucleotide sequence of it and the flanking region analyzed by direct sequencing of the inverse PCR product, an expression vector was constructed. The E. coli cells harboring the plasmid produced an about 80-kDa protein having pullulanase activity, the optimum temperature of which was 70℃.
- 社団法人日本農芸化学会の論文
- 2001-09-23
著者
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SAKAI Hiroshi
Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University
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Yasuda Michiko
Environmental Molecular Biology Laboratory Discovery Research Institute Riken
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Yasuda Michiko
Plant-endophyte Interactions Laboratory Center For Intellectual Property Strategies Riken
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TONOZUKA Takashi
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Sakai H
Okayama Univ. Okayama Jpn
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Yasuda M
Environmental Molecular Biology Laboratory Discovery Research Institute Riken
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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TOMIYASU Kanae
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc
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YATO Kumiko
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc
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YASUDA Megumi
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc
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IBUKA Akiko
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc
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Yato Kumiko
Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science
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Ibuka Akiko
Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science
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Yasuda Megumi
Department Of Anatomy Division 1 Kinki University School Of Medicine
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Tomiyasu Kanae
Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Tonozuka Takashi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Yasuda Michiko
Plant Acquired Immunity Research Unit Discovery Research Institute Riken
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Sakai Hiroshi
Department Of Agricultural Chemistry The University Of Tokyo
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Tonozuka Takashi
Forage Crop Breeding Unit National Agricultural Research Center For Kyushu Okinawa Region
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Ibuka Akiko
Department of Biotechnology, University of Tokyo
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