Cloning and Nucleotide Sequence of the Pullulanase Gene of Thermus thermophilus HB8 and Production of the Enzyme in Escherichia coli(Biochemistry & Molecular Biology)

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概要

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• A 3.4-kb Sph1 fragment carrying the pullulanase gene of Thermus thermophilus HB8 was cloned. Based on the nucleotide sequence of it and the flanking region analyzed by direct sequencing of the inverse PCR product, an expression vector was constructed. The E. coli cells harboring the plasmid produced an about 80-kDa protein having pullulanase activity, the optimum temperature of which was 70℃.

• 社団法人日本農芸化学会の論文
• 2001-09-23

著者

• SAKAI Hiroshi

  Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University

• Yasuda Michiko

  Environmental Molecular Biology Laboratory Discovery Research Institute Riken

• Yasuda Michiko

  Plant-endophyte Interactions Laboratory Center For Intellectual Property Strategies Riken

• TONOZUKA Takashi

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• Sakai H

  Okayama Univ. Okayama Jpn

• Yasuda M

  Environmental Molecular Biology Laboratory Discovery Research Institute Riken

• Sakai H

  Department Of Bioscience And Biotechnology Okayama University

• TOMIYASU Kanae

  Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc

• YATO Kumiko

  Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc

• YASUDA Megumi

  Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc

• IBUKA Akiko

  Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Scienc

• Yato Kumiko

  Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science

• Ibuka Akiko

  Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science

• Yasuda Megumi

  Department Of Anatomy Division 1 Kinki University School Of Medicine

• Tomiyasu Kanae

  Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science

• Sakai H

  Department Of Pharmacology Nagasaki University Shool Of Dentistry

• Tonozuka Takashi

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Yasuda Michiko

  Plant Acquired Immunity Research Unit Discovery Research Institute Riken

• Sakai Hiroshi

  Department Of Agricultural Chemistry The University Of Tokyo

• Tonozuka Takashi

  Forage Crop Breeding Unit National Agricultural Research Center For Kyushu Okinawa Region

• Ibuka Akiko

  Department of Biotechnology, University of Tokyo

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