Structures of Thermoactinomyces vulgaris R-47 α-Amylase II Complexed with Substrate Analogues

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概要

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• The structures of Thermoactinomyces vulgaris R-47 α-amylase II mutant (d325nTVA II) complexed with substrate analogues, methyl β-cyclodextrin (mβ-CD) and maltohexaose (G6), were solved by X-ray diffraction at 3.2Å and 3.3Å resolution, respectively. In d325nTVA II-m β-CD complex, the orientation and binding-position of β-CD in TVA II were identical to those in cyclodextin glucanotransferase (CGTase). The active site residues were essentialy conserved, while there are no residues corresponding to Tyr89,Phe183,and His233 of CGTase in TVA II. In d325nTVA II-G6 complex, the electron density maps of two glucosyl units at the non-reducing end were disordered and invisible. The four glucosyl units of G6 were bound to TVA II as in CGTase, while the others were not stacked and were probably flexible. The residues of TVA II corresponding to Tyr89,Lys232,and His233 of CGTase were completely lacking. These results suggest that the lack of the residues related to α-glucan and CD-stacking causes the functional distinctions between CGTase and TVA II.

• 2001-03-23

著者

• 神鳥 成弘

  東京農工大学工学部生命工学科

• TONOZUKA Takashi

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• 殿塚 隆史

  東京農工大学農学部

• Yokota T

  Faculty Of Agriculture Shizuoka University

• YOKOTA Takehiro

  Department of Applied Biological Science, Tokyo University of Agriculture Technology

• SHIMURA Yoichiro

  Department of Applied Biological Science, Tokyo University of Agriculture Technology

• ICHIKAWA Kazuhiro

  Department of Applied Biological Science, Tokyo University of Agriculture Technology

• KAMITORI Shigehiro

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• SAKANO Yoshiyuki

  Department of Applied Biological Science, Tokyo University of Agriculture Technology

• Yuuki T

  Asahi Breweries Ltd. Ibaraki Jpn

• Tonozuka Takashi

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Ichikawa Kimihisa

  Department Of Applied Microbiology Nippon Roche Research Center

• Shimura Y

  Kitasato Univ. School Of Medicine Kanagawa Jpn

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• 神鳥 成弘

  Department Of Biotechnology & Life Science Tokyo University Of Agriculture & Technology

• Yokota Takao

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Tonozuka Takashi

  Forage Crop Breeding Unit National Agricultural Research Center For Kyushu Okinawa Region

• Ichikawa Kazuhiro

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

• SAKANO Yoshiyuki

  Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University

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