Characterization of Rat N-Acetylglucosaminyltransferase I Expressed in Esherichia coli
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概要
- 論文の詳細を見る
The catalytic domain (sGnT-I) of rat liver N-acetylglucosaminyl-transferase I (GnT-I) was expressed in Escherichia coli. Lysates from pETsGnT-I transformants contained a prominent protein species of 46kDa with which a significant GnT-I activity was associated. To purify the relevant enzyme, we constructed cDNAs encoding sGnT-ICH and sGnT-INH, which had six additional histi-dine residues as an affinity tag at the C-terminal and the N-terminal of sGnT-I, respectively, and introduced them into E. coli cells for expression. sGnT-INH was purified and its enzymatic properties were examined.
- 社団法人日本農芸化学会の論文
- 1995-09-23
著者
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Komano T
Kyoto Univ. Kyoto Jpn
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano Tohru
京都大学
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Kioka N
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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Kioka Noriyuki
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Kioka Noriyuki
大阪府立大学 農学生命科研究
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Kioka Noriyuki
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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SAKAI Hiroshi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Sakai H
Okayama Univ. Okayama Jpn
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Fukada T
Wafermasters Inc. Ca Usa
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Fukada Takashi
Wafer Masters Inc.
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Fukada Takashi
Laboratory Of Applied Molecular Biology Division Of Applied Biochemistry Graduate School Of Agricult
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Fukada Takashi
Laboratory Of Applied Molecular Biology Department Of Applied Biochemistry Osaka Prefecture Universi
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Nishiu Jun
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Fukada Takashi
Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.
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Nishiu Jun
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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Sakai Hiroshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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