Expression of Human P-Glycoprotein in Yeast Cells : Effects of Membrane Component Sterols on the Activity of P-Glycoprotein(Biological Chemistry)
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概要
- 論文の詳細を見る
A human MDRI cDNA was introduced into yeast cells. Immunoblot analysis and indirect immunostaining showed that some of the P-glycoprotein produced was situated in its native orientation in the yeast plasma membrane. Drug-binding activities of the recombinant P-glycoproteins were markedly decreased compared to that of the authentic P-glycoprotein. To identify the bases of decreased binding we studied the effects of membrane component sterols on the azidopine binding and found that ergosterol, which is the main sterol in the yeast membrane, and calciferol, which is produced from ergosterol by UV irradiation, inhibited azidopine binding. These sterols in yeast membrane probably inhibit the function of human P-glycoprotein as a multidrug transporter in yeast cells, because expression of P-glycoprotein in yeast cells did not confer resistance to doxorubicin.
- 社団法人日本農芸化学会の論文
- 1991-07-23
著者
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Komano T
Kyoto Univ. Kyoto Jpn
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano Tohru
京都大学
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Saeki T
Kyoto Univ. Kyoto Jpn
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Ueda Kazumitsu
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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SHIBANO YUJI
Suntory Research Center
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Saeki Tohru
Laboratory of Molecular Nutrition, Kyoto Prefectural University
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Ueda Kazumitsu
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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SHIMABUKU Alfredo
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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AZUMA Yoshinao
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Saeki Tohru
Laboratory Of Molecular Nutrition Department Of Biological Resource Chemistry Kyoto Prefectural Univ
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Shibano Y
Suntory Ltd. Osaka
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Azuma Yoshinao
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Shibano Yuji
Suntory Limited
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Saeki Tohru
Laboratory Of Molecular Nutrition Kyoto Prefectural University
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Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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Shimabuku Alfredo
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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