Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts : A Quick Method for Detection of Foreign Gene Expression in mRNA Level
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概要
- 論文の詳細を見る
We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24h after electroporation mediated DNA transfer.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Sakai Fukumi
Wood Research Institute, Kyoto University
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SAKAI Hiroshi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Sakai F
Kyoto Univ.
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Kuroda H
Kyoto Univ.
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Kuroda H
Laboratory Of Gene Expression Wood Research Institute Kyoto University
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Kuroda Hiroyuki
Wood Research Institute Kyoto University
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Sakai Fukumi
Wood Research Institute Kyoto University
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Qiao Jingbo
Wood Research Institute Kyoto University
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Ishihara Yasuyuki
Laboratory Of Gene Expression Wood Research Institute Kyoto University
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ISHIHARA Yasuyuki
Wood Research Institute, Kyoto University
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Sakai Hiroshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Kuroda Hiroyuki
Wood Res. Inst. Kyoto Univ.
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