Effects of Bacillus thuringiensis var.israelensis 20-kDa Protein on Production of the Bti 130-kDa Crystal Protein in Escherichia coli
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概要
- 論文の詳細を見る
A 20-kDa protein of Bacillus thuringiensis var. israelensis (Bti) has been shown to be necessary for the efficient expression of the 27-kDa mosquitocidal protein gene in Escherichia coli. We have investigated the effects of this 20-kDa protein on the expression of two 130-kDa mosquitocidal protein genes (cryIV A, cryIV B) in E.coli by supplying the 20-kDa protein gene in trans. When a recombinant plasmid, pLH4BX, which was constructed to express cryIV A under the E.coli lac promoter on pUC19,coexisted with the 20-kDa protein gene, a striking increase in production of the fused CryIV A was detected. This was not accompanied by an increase in the amount of intracellular mRNA, suggesting that 20-kDa protein exerts a posttranscriptional effect. We conclude that the 20-kDa protein also stimulates the production of 130-kDa protein in E.Coli.
- 社団法人日本農芸化学会の論文
- 1992-09-23
著者
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Komano T
Kyoto Univ. Kyoto Jpn
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano Tohru
京都大学
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Yoshida Ken-ichi
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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SAKAI Hiroshi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Sakai H
Okayama Univ. Okayama Jpn
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Sen Kikuo
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
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YOSHISUE Hajime
Laboratory to Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Yoshisue H
Kyowa Hakko Kogyo Co. Ltd. Shizuoka Jpn
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Yoshisue Hajime
Laboratory To Biochemistry Department Of Agricultural Chemistry Kyoto University
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Yoshida K
Tohoku Univ. Sendai‐shi Jpn
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Sen K
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Sen Kikuo
Department Of Bioscience And Biotechnology Shinshu University
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Yoshida Ken-ichi
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Yoshida Ken-ichi
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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Sakai Hiroshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Sen Kikuo
Department of Bioscience and Biotechnology Faculty of Agriculture, Shinshu University
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