Production of a Site Specifically Cleavable P-Glycoprotein-β-galactosidase Fusion Protein(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
We have fused full length and the carboxyl-half of human MDRI cDNA with the E. coli IacZ gene via a collagen linker and allowed their expression in yeast Saccharomyces cerevisiae. Using antibodies against β-galactosidase we partially purified the fusion proteins by immunoprecipitation and show here that the full length fusion protein has ATPase activity. By contrast, the fusion protein containing the carboxyl-half of P-glycoprotein did not show ATPase activity, indicating that both domains of P-glycoprotein are necessary. By treatment of the immunoprecipitated fusion protein with collagenase, P-glycoprotein was released from the β-galactosidase moiety. The results shown here open the possibility for a large scale purification of P-glycoprotein using this site specifically cleavable fusion protein.
- 社団法人日本農芸化学会の論文
- 1991-04-23
著者
-
Komano T
Kyoto Univ. Kyoto Jpn
-
Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
-
Komano Tohru
京都大学
-
Saeki T
Kyoto Univ. Kyoto Jpn
-
Ueda Kazumitsu
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
-
Saeki Tohru
Laboratory of Molecular Nutrition, Kyoto Prefectural University
-
Ueda Kazumitsu
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
-
SHIMABUKU Alfredo
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
-
Saeki Tohru
Laboratory Of Molecular Nutrition Department Of Biological Resource Chemistry Kyoto Prefectural Univ
-
Saeki Tohru
Laboratory Of Molecular Nutrition Kyoto Prefectural University
-
Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
-
Shimabuku Alfredo
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
関連論文
- 2E11-4 ポリスチレン親和性ペプチドを用いるタンパク質吸着の配向制御(生物化学工学,一般講演)
- 食品製造プロセスにおける新規洗浄方法の原理とその特性 (特集 食品工場の洗浄・除菌技術)
- S219 マイクロバイオリアクターとマイクロ生体反応素子の展望と課題(コンビナトリアル・バイオエンジニアリングの進展,シンポジウム)
- 306 二糖-高分子複合アモルファスマトリクスの諸物性の解析(培養工学,一般講演)
- 308 酵素の固体表面に対する付着特性の解析と付着の制御(培養工学,一般講演)
- 309 機能性タンパク質の固体表面への結合制御に関する基礎的研究(培養工学,一般講演)
- 310 H_2O_電気分解処理における金属表面からのタンパク質汚れの除去(培養工学,一般講演)
- 549 Streptomyces sp.由来新規アシラーゼの多様な基質特異性(酵素・酵素工学・タンパク質工学,一般講演)
- 食品製造プロセスで発生する汚れの新規洗浄方法 (特集:食品製造装置の洗浄殺菌)
- 656 H_2O_2-電気分解処理によるステンレス表面に付着したタンパク質汚れの除去
- 655 キトサン/コンドロイチン硫酸複合ゲルのpH応答型膨潤特性とその応用
- 413 膜面液体培養法を用いた遺伝子組換えAspergillus oryzaeによる酵素生産
- 247 Streptomyces sp.由来カプサイシン加水分解酵素の精製
- 246 トランスグルタミナーゼによるβ-ラクトグロブリンの脂肪酸修飾
- Adsorption Characteristics of Oligopeptides Composed of Acidic and Basic Amino Acids on Titanium Surface(BIOCHEMICAL ENGINEERING)
- Protease Susceptibility of β-Lactoglobulin Adsorbed on Stainless Steel Surface as Evidence of Contribution of Its Specific Segment to Adsorption(MISCELLANEOUS)
- Cleaning of a Stainless Steel Surface Fouled with Protein Using a UV-H_2O_2 Technique
- Detection of ABCA7-positive cells in salivary glands from patients with Sjogren's syndrome
- Characteristics of α-Glucosidase Production from Recombinant Aspergillus oryzae by Membrane-Surface Liquid Culture in Comparison with Various Cultivation Methods(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Production of Nonproteinaceous Amino Acids Using Recombinant Escherichia coli Cells Expressing Cysteine synthase and Related Enzymes with or without the Secretion of O-Acetyl-L-Serine (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- サーモライシンの有機溶媒への懸濁方法が酵素活性に及ぼす影響
- Structural Analysis of Repetitive DNA Sequences in the Goat Growth Hormone Gene Region(Biological Chemistry)
- Cloning and Sequencing of Goat Growth Hormone Gene(Biological Chemistry)
- Mechanism of multidrug recognition by MDR1/ABCB1
- ABC proteins : key molecules for lipid homeostasis
- Inhibitory Effects of a Cyclosporin Derivative, SDZ PSC 833, on Transport of Doxorubicin and Vinblastine via Human P-Glycoprotein
- Tissue- and Development-specific Expression of Goat Insulin-like Growth Factor-I (IGF-I) mRNAs
- Dynamic Aspects in the Expression of the Goat Insulin-like Growth Factor-I (IGF-I)Gene : Diversity in Transcription and Post-transcription
- Binding of an Engineered 130-kDa Insecticidal Protein of Bacillus thuringiensis var.israelensis to Insect Cell Lines
- Importance of the Central Region of 130-kDa Insecticidal Proteins of Bacillus thuringiensis var. israelensis for Their Activity in Vivo and in Vitro
- Effects of Bacillus thuringiensis var.israelensis 20-kDa Protein on Production of the Bti 130-kDa Crystal Protein in Escherichia coli
- Expression of the Two 130-kDa Protein Genes of Bacillus thuringiensis var. israelensis in Bacillus subtilis(Biological Chemistry)
- Insecticidal Activity of a Peptide Containing the 30th to 695th Amino Acid Residues of the 130-kDa Protein of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Insecticidal Activity of the Products of Two 130-kDa Protein Genes(Biological Chemistry)
- Cloning and Nucleotide Sequences of the Two 130 kDa Insecticidal Protein Genes of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Application of Vanadate-Induced Nucleotide Trapping to Plant Cells for Detection of ABC Proteins
- Evidence for the Existence of a Soybean Resistant Protein That Captures Bile Acid and Stimulates Its Fecal Excretion
- Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr130–Pro142) of the Highly Conserved Region of Mouse Slc10a2
- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na+-Dependent Bile Acid Transporters
- Vinexin, CAP/ponsin, ArgBP2 : a Novel Adaptor Protein Family Regulating Cytoskeletal Organization and Signal Transduction
- Genomic Structure and 5'-Flanking Sequences of Rat N-Acetylglucosaminyltransferase I Gene and Regulatory Role of Its Transcriptional Diversity(Biochemistry & Molecular Biology)
- Characterization of Rat N-Acetylglucosaminyltransferase I Expressed in Esherichia coli
- Cloning of a cDNA Encoding N-Acetylglucosaminyltransferase I from Rat Liver and Analysis of Its Expression in Rat Tissues
- Synthesis of (R)-Enantiomers of 5-Hexadecanolide and 4-Dodecanolide, Pheromone of the Oriental Hornet and a Defensive Secretion of Rove Beetles, Employing Asymmetric Reduction with Immobilized Baker's Yeast(Organic Chemistry)
- Molecular Mechanisms of Subcellular Localization of ABCG5 and ABCG8
- Expression of Human P-Glycoprotein in Yeast Cells : Effects of Membrane Component Sterols on the Activity of P-Glycoprotein(Biological Chemistry)
- Production of a Site Specifically Cleavable P-Glycoprotein-β-galactosidase Fusion Protein(Biological Chemistry)
- The Effects of Metal Ions on the DNA Damage Induced by Hydrogen Peroxide(Biological Chemistry)
- Alternative Secondary Structures in the Phage G4 Origin of the Complementary DNA Strand Synthesis : Effects of NaCl Concentration on the Bleomycin-DNA Interaction
- Volume-Sensitive Cl^- Channel in Human Epithelial Cells : Regulation by ATP and Relation to P-Glycoprotein
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate (2):Importance of the P2 and P4 Residues
- Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts : A Quick Method for Detection of Foreign Gene Expression in mRNA Level
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate
- Mutational Analysis of the Putative Substrate-binding Site of 3C Proteinase of Coxsackievirus B3
- Expression and Purification of Recombinant 3C Proteinase of Coxsackievirus B3
- Nucleotide Sequence of the Glycerol-3-phosphate Dehydrogenase Gene of Escherichia coli and Regulation by the cAMP-CRP Complex(Biological Chemistry)
- Soybean Resistant Proteins Interrupt an Enterohepatic Circulation of Bile Acids and Suppress Liver Tumorigenesis Induced by Azoxymethane and Dietary Deoxycholate in Rats
- Non-involvement of the K-ras Mutation in Colon Carcinogenesis Promoted by Dietary Deoxycholate in Azoxymethane-treated Rats
- Plasmids and Insecticidal Activity of Delta-endotoxin Crystals from Bacillus thuringiensis var. israelensis
- Mechanism of Action of Bacillus thuringiensis Insecticidal Delta-endotoxin on Insect Cells in Vitro
- Functional Distinction among Structural Subsections in the Specific Priming Signal for DNA Replication of the Broad Host-Range Plasmid RSF1010
- GROWTH REGULATION BY cAMP IN ESCHERICHIA COLI
- Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate
- Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate
- Effects of cya and cid Mutations on Cell Growth in Escherichia coli(Biological Chemistry)
- Molecular Mechanisms of Subcellular Localization of ABCGS and ABCG8
- Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr^-Pro^) of the Highly Conserved Region of Mouse Slc10a2
- Soybean Resistant Protein Elevates Fecal Excretion of Cholesterol and Bile Acids and Decreases Hepatic Cholesterol Content in Comparison with Soybean Protein Isolate
- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na^+-Dependent Bile Acid Transporters
- Taurocholic Acid Does Not Induce Apoptosis in HCT116 Cells Regardless of Its Intracellular Concentration
- Characteristics of Apoptosis in HCT116 Colon Cancer Cells Induced by Deoxycholic Acid
- Inverse Correlation between the Nitrogen Balance and Induction of Rat Liver Serine Dehydratase (SDH) by Dietary Protein
- Postprandial Changes in Portal Venous Free Amino Acids and Insulin/Glucagon Ratios as the Result of Protein Over-Intake Are Not Directly Linked to Serine Dehydratase Induction in Rat Liver Irrespective of Age
- Response of the Induction of Rat Liver Serine Dehydratase to Changes in the Dietary Protein Requirement(Food & Nutrition Science)
- Quantity as Well as Quality of Dietary Protein Affects Serine Dehydratase Gene Expression in Rat Liver
- Characterization of Cloned Mouse Na^+/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells(Biochemistry & Molecular Biology)
- Effects of Cys Mutation on Taurocholic Acid Transport by Mouse Ileal and Hepatic Sodium-dependent Bile Acid Transporters(Biochemistry & Molecular Biology)
- Antitumorigenic Effects of Several Food Proteins in a Rat Model with Colon Cancer and Their Reverse Correlation with Plasma Bile Acid Concentration
- Preventive Effect of Soybean Resistant Proteins against Experimental Tumorigeneses in Rat Colon
- Characterization, cDNA Cloning, and Fuctional Expression of Mouse Ileal Sodium-Dependent Bile Acid Transporter
- Effect of Polyethylene Glycol in Plasmid DNA Solution on Transformation of CaCl2-Treated Escherichia coli Cells
- Quantity as Well as Quality of Dietary Protein Affects Serine Dehydratase Gene Expression in Rat Liver
- Effects of Detergents and Divalent Cations on the Functioning of Cell Envelopes of Escherichia coli in the Early Stages of Infection with Bacteriophage øX174
- DNA Replication of IncQ Broad-host-range Plasmids in Gram-negative Bacteria
- A Simple Method for Isolation of Chloroplast DNA from Marchantia polymorpha L. Cell Suspension Cultures
- In Vitro DNA Synthesis by Chloroplasts Isolated from Marchantia polymorpha L. Cell Suspension Cultures
- DNA Strand Breakage In Vitro by Autoxidized Unsaturated Fatty Acids
- The Mechanism of Inactivation of Bacteriophage ØX174 by Anthracycline Antibiotics
- Induction of Strand Breakage in φX174 RFI DNA by Aminosugar Derivatives
- Molecular Cloning of Promoters Functional in Escherichia coli from Chloroplast DNA of a Liverwort, Marchantia polymorpha
- Induction of Single Strand Scission in φX174 RFI DNA by D-Isoglucosamine
- Inactivation of Escherichia coli Phages by Sugar Phosphates
- Actions of Mitomycin C Reduced with Sodium Borohydride on Bacteriophage ΦX174 and Its Single- and Double-stranded DNAs
- Involvement of a Cell Envelope Component of Escherichia coli in the Early Stages of Infection with Bacteriophage φX174
- DNA Products in In Vitro DNA Synthesis Using Bacteriophage ΦX174 Single-stranded DNA
- Studies on the Functional Relationship between φX174 and SI3 Coded Proteins Using an In Vitro DNA Synthesis System