Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and Comparison of the Properties of the Enzymes Produced in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
The genes for the pyruvate kinases of a psychrophile, Bacillus psychrophilus, and a mesophile, Bacillus licheniformis, have been cloned in Escherichia coli, and all their nucleotides were sequenced. The two bacterial enzymes each had an extra C-terminal sequence consisting of about 110 amino acid residues, which has been found in the B. stearothermophilus enzyme. Both enzymes were overexpressed in E. coli and the properties of the purified enzymes were compared to those of the B. stearothermophilus enzyme. Both enzymes were less stable than the B. stearothermophilus one. The B. psychrophilus enzyme was more stable than the B. licheniformis one. Similarly to the B. licheniformis and B. stearothermophilus pyruvate kinases, the B. psychrophilus enzyme was activated by AMP or ribose 5-phosphate, and inhibited by ATP or fructose 1,6-bisphosphate. Thus, these enzymes were very similar in the sigmoidal saturation curve for phosphoenolpyruvate and allosteric effectors, but their optimum temperatures and thermostabilities were very different.
- 社団法人日本農芸化学会の論文
- 1995-08-23
著者
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SAKAI Hiroshi
Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University
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TANAKA Kenichi
Department of Physics, School of Medicine, Sapporo Medical University
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Ohta T
Hokkaido Food Processing Res. Center Hokkaido Jpn
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Sakai Hideo
University Forest In Chichibu Graduate School Of Agricultural And Life Sciences The University Of To
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Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
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MATSUZAWA Hiroshi
Department of Biotechnology, The University of Tokyo
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Sakai H
Okayama Univ. Okayama Jpn
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OHTA Takahisa
Department of Agricultural Chemistry, The University of Tokyo
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Ohta Takahisa
Department Of Agricultural Chemistry The University Of Tokyo
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
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Tanaka Kenichi
Department Of Physics School Of Medicine Sapporo Medical University
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Tanaka Kenichi
Department Of Electronic And Bioinformatics School Of Science Technology Meiji University
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Matsuzawa Hiroshi
Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo
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Sakai Hiroshi
Department Of Agricultural Chemistry The University Of Tokyo
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Tanaka Kenichi
Department Of Anesthesiology Emory University School Of Medicine
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Tanaka Kenichi
Department Of Biotechnology The University Of Tokyo
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Matsuzawa Hiroshi
Department of Bioscience and Biotechnology, Aomori University
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MATSUZAWA Hiroshi
Department of Agricultural Chemistry, The University of Tokyo
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