Identification of Monoterpene Alcohol β-Glucosides in Sweet Potatoes and Purification of a Shiro-koji β-Glucosidase(Food & Nutrition)
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概要
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Linalyl-β-glucoside (LBG), α-terpinyl-β-glucoside (TBG), neryl-β-glucoside (NBG), and geranyl-β-glucoside (GBG) in sweet potatoes were identified as TMS derivatives from their MS fragmentation patterns and retention times. Amounts of these monoterpene alcohol-β-glucosides were from 36.9 μg/kg sweet potato of LBG to 189.7 μg/kg sweet potato of TBG. On the other hand, 75.8 μg/l distillation residue of TBG and traces of other monoterpene alcohol-β-glucosides occurred in the distillation residue. β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) in shiro-koji were purified using p-nitrophenyl-β-glucoside (PNPG), and three active fractions, one major fraction and two very minor fractions, were found. The Km against GBB was 2.4mM and the Ki against glucose was 7.6 mM. Relative activity in the 20% ethanol solution was 68%. Shiro-koji β-glucosidases were active on tested β-glucosides except for LBG and TBG.
- 社団法人日本農芸化学会の論文
- 1991-07-23
著者
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Ohta T
Hokkaido Food Processing Res. Center Hokkaido Jpn
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OHTA TAKEO
National Research Institute of Brewing
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SAMUTA TAKASHI
National Research Institute of Brewing
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OHBA TOSHITERU
National Research Institute of Brewing
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Omori Toshiro
National Research Institute Of Brewing
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SHIMOJO Hirokazu
National Research Institute of Brewing
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HASHIMOTO Kenji
National Research Institute of Brewing
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Ohta T
Numazu Industrial Res. Inst. Shizuoka Prefecture Numazu‐shi Jpn
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Samuta T
National Research Institute Of Brewing
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