Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1 : Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg

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概要

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• Aqualysin I is the alkaline serine protease isolated from an extreme thermophile, Thermus aquaticus YT-1. We analyzed kinetic properties of aqualysin I, using sixteen kinds of chromogenic succinyl-tripeptide p-nitroanilides as substrates. And we compared the substrate specificity of aqualysin I with those of proteinase K, subtilisin BPN', and subtilisin Carlsberg. We found that aqualysin I had three subsites, S1,S2,and S3,in the substrate binding site. S1 site preferred alanine and phenylalanine. S2 site preferred alanine and norleucine. And S3 site preferred phenylalanine and isoleucine. These specificities were similar to those of proteinase K and subtilisin BPN'. The specificity of subtilisin Carlsberg differed from those of other enzymes.

• 社団法人日本農芸化学会の論文
• 1998-11-23

著者

• Ohta T

  Hokkaido Food Processing Res. Center Hokkaido Jpn

• Tanaka T

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka T

  Hiroshima Univ. Higashi Hiroshima Jpn

• Mori Hiroaki

  Department Of Agricultural Chemistry The University Of Tokyo

• MATSUZAWA Hiroshi

  Department of Biotechnology, The University of Tokyo

• OHTA Takahisa

  Department of Agricultural Chemistry, The University of Tokyo

• Tanaka T

  Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center

• Tanaka Takaaki

  Graduate School Of Science And Technology Niigata University

• Ohta Takahisa

  Department Of Agricultural Chemistry The University Of Tokyo

• Matsuzawa H

  Department Of Bioscience And Biotechnology Aomori University

• TANAKA Terumichi

  Department of Biotechnology, The University of Tokyo

• Tanaka T

  Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University

• Tanaka Takaaki

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Tanaka Terumichi

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• Matsuzawa Hiroshi

  Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo

• Matsuzawa Hiroshi

  Department of Bioscience and Biotechnology, Aomori University

• MATSUZAWA Hiroshi

  Department of Agricultural Chemistry, The University of Tokyo

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