Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
We purified and characterized an aminopeptidase from Streptococcus thermophilus YRC001 to obtain an enzyme for the application of reducing bitter-defect in cheese manufacturing. The purified enzyme was a monomer, and its molecular mass was estimated to be 90-100 kDa. It had a broad substrate specificity, and mostly hydrolyzed lysyl and leucyl peptides. The optimal temperature and pH for the enzyme were 35℃ and pH 6.5, respectively. EDTA, o-phenanthroline, and p-chloromercuribenzoate inhibited its activity, therefore it was considered to be a metallopeptidase. The purified enzyme efficiently reduced the bitterness of a trypsin digest of reconstituted skim milk. Therefore, we cloned a gene for the enzyme from YRC001. The nucleotide sequence of a 2,940-bp Xbal fragment containing the gene was analyzed. The gene encoded 849 amino acids, and the calculated molecular mass for the mature enzyme (initial methionine is removed) was 96,434. The deduced amino acid sequence showed high homology with the known bacterial lysyl aminopeptidase (aminopeptidase N).
- 社団法人日本農芸化学会の論文
- 2003-04-23
著者
-
Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
-
KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
-
MOTOSHIMA Hidemasa
Research Center, Yotsuba Milk Products Co., Ltd.
-
Kaminogawa Shuichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
-
Tsukasaki F
Research Center Yotsuba Milk Products Co. Ltd.
-
Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
-
SHIRAISHI Takayasu
Research Center, Yotsuba Milk Products, Co., Ltd.
-
TSUKASAKI Fuji
Research Center, Yotsuba Milk Products, Co., Ltd.
-
Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
-
Motoshima Hidemasa
Research Center Yotsuba Milk Products Co. Ltd.
-
Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
Shiraishi Takayasu
Research Center Yotsuba Milk Products Co. Ltd.
-
Tsukasaki Fuji
Research Center Yotsuba Milk Products Co. Ltd.
-
Motoshima Hidemasa
Research and Development Department, Yotsuba Milk Products Co.
関連論文
- Antigenicity of Modified β-Lactoglobulin Examined by Three Different Assays
- Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer’s Patches
- Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
- Characteristic Intestinal Microflora of Specific Pathogen-Free Mice Bred in Two Different Colonies and their Influence on Postnatal Murine Immunocyte Profiles
- Thermostable β-Galactosidase from an Extreme Thermophile, Thermus sp. A4 : Enzyme Purification and Characterization, and Gene Cloning and Sequencing
- APOPTOTIC SIGNAL AT THE FINAL STAGE OF GASTRULATION IN SEA URCHIN EMBRYO(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- CHANGES IN THE CELL NUMBER OF SEA URCHIN EMBRYOS AND DETECTION OF APOPTOTIC CELLS BY COMETASSAY(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Oral administration of freeze-dried kefir reduces intestinal permeation of and oral sensitization to ovalbumin in mice
- DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
- DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
- Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
- Stimulatory Effect of Human Lactoferrin on DNA Synthesis in BALB/c 3T3 Cells
- サトウキビ抽出液のブタ免疫応答調節能(免疫学)
- Role of Olfaction in Food Preference as Evaluated in an Animal Model
- Apoptosis of Antigen-specific T Cells Induced by Oral Administration of Antigen : Comparison of Intestinal and Non-intestinal Immune Organs
- Carboxypeptidase Taq, a Thermostable Zinc Enzyme, from Thermus aquaticus YT-1 : Molecular Cloning, Sequencing, and Expression of the Encoding Gene in Escherichia coli
- Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
- Vaccination with an immunodominant peptide of bovine type II collagen induces an anti-TCR response, and modulates the onset and severity of collagen-induced arthritis
- Suppression of Collagen-Induced Arthritis in DBA/1J Mice by Preimmunization with House Dust Mite Extract
- Enhancing Effect of Interleukin-4 on the Secretion of Interferon-γ by α_-Casein-specific CD8^+ T Cells
- α_-Casein-specific CD8^+ T Cell Clone That Inhibits Its Own Interferon-γ Production by Producing Interleukin-10
- Amino Acid Residue Substitution at T-Cell Determinant-flanking Sites in β-Lactoglobulin Modulates Antigen Presentation to T Cells through Subtle Conformational Change(Food & Nutrition Science)
- In Vivo Macrophage-stimulation Activity of the Enzyme-degraded Water-soluble Polysaccharide Fraction from a Marine Alga (Gracilaria verrucosa)
- Macrophage Stimulation Activity of the Polysaccharide Fraction from a Marine Alga (Porphyra yezoensis):Structure-Function Relationships and Improved Solubility
- Activation of Murine Macrophages by Polysaccharide Fractions from Marine Algae (Porphyra yezoensis)
- Antigen Feeding Increases Frequency and Antigen-specific Proliferation Ability of Intraepithelial CD4^+ T Cells in αβ T Cell Receptor Transgenic Mice(Food & Nutrition Science)
- Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells(Food & Nutrition Science)
- Characterization of a Water-soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4
- Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
- Strong Influence of the Processing of the Antigen on Negative Effects on T Cell Activation by Regions Outside the Determinant Area of Bovine α_-Casein
- Production and Characterization of Anti-human Insulin Antibodies in the Hen's Egg(Biological Chemistry)
- Structure of α_-Casein at an Oil/Water Interface: An Analytical Approach Using Immunochemical Methods(Biological Chemistry)
- Methionine Oxidation and Proline cis-trans Isomerization in α_-Casein on Emulsification with Oxidized Lipid(Biological Chemistry)
- In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine
- Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
- Analysis of Cytokine Producing Activity of Intestinal Intraepithelial T Cells from TCR β-Chain and δ-Chain Mutant Mice
- PDE4 inhibitor (GPD-1116) prevents senescence-accelerated mice P1 strain (SAMP1) from developing cigarette smoke-induced emphysema.(Annual Report from the Division of Biomedical Research Resources, Juntendo University Graduate School of Medicine)
- Characterization and Expression Analysis of the Exopolysaccharide Gene Cluster in Lactobacillus fermentum TDS030603
- Low Temperature Sensitization in the Weld Metal of Type 308 Stainless Steel and Its Improvement by Laser Surface Melting Treatment
- Reddish Escherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporphyrinogen III Methylase : Cloning, Sequencing, and Expression of the Gene
- Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and Comparison of the Properties of the Enzymes Produced in Escherichia coli
- Dietary Nucleotides Increase the Proportion of a TCRγδ^+ Subset of Intraepithelial Lymphocytes (IEL) AND IL-7 Production by Intestinal Epithelial Cells (IEC) : Implications for Modification of Cellular and Molecular Cross-talk between IEL and IEC by Dieta
- Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae(Food & Nutrition)
- Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
- Food allergens and mucosal immune systems with special reference to recognition of food allergens by gut-associated lymphoid tissue
- Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
- Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
- Comparison of pH and Ionic Strength Dependence of Interactions between Monoclonal Antibodies and Bovineβ-Lactoglobulin
- Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells
- Inhibitory Effect of Lactobacillus gasseri TMC0356 and Lactobacillus GG on Enhanced Vascular Permeability of Nasal Mucosa in Experimental Allergic Rhinitis of Rats
- Role of Two α-L-Arabinofuranosidases in Arabinoxylan Degradation and Characteristics of the Encoding Genes from Shochu Koji Molds, Aspergillus kawachii and Aspergillus awamori(BREWING AND FOOD TECHNOLOGY)
- Purification and Characterization of a Feruloylesterase from Aspergillus awamori
- PE-429 Safety and Efficacy of Drug Eluting Stent in Patients with Acute Coronary Syndrome(PE072,Coronary Revascularization, PCI (DES) 7 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Society)
- Application of a Metal Switch to Aqualysin I, a Subtilisin-type Bacterial Serine Protease, to the S3 Site Residues, Ser102 and Gly131
- Identification and Designing of the S3 Site of Aqualysin I, a Thermophilic Subtilisin-Related Serine Protease
- Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO
- Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1 : Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg
- P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor
- Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1
- Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
- T Cell Response to Hen's Egg Ovomucoid(Biological Chemistry)
- Comparison of Four Distinct Idiotypes of Monoclonal Antibodies against Hen's Egg Ovomucoid(Biological Chemistry)
- Content and Chemical Compositions of Cerebrosides in Lactose-assimilating Yeasts
- Effects of Growth Temperature on Cerebroside Content and Chemical Composition in Kluyveromyces lactis
- Suppression of the Systemic Immune Response to Casein by Oral Administration of a Tryptic Digest of Casein
- Involvement of NH_2-Terminal Pro-sequence in the Production of Active Aqualysin I (a Thermophilic Serine Protease) in Escherichia coli(Biological Chemistry)
- High-pressure Proteolytic Digestion of Food Proteins : Selective Elimination of β-Lactoglobulin in Bovine Milk Whey Concentrate(Food & Nutrition)
- Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
- Occurrence of High Molecular Weight EGF Complexes in Human Milk(Food & Nutrition)
- Preparation of Monoclonal Antibody against Bovine β-Lactoglobulin and Its Unique Binding Affinity(Biological Chemistry)
- Emulsifying Properties of Peptides Obtained from the Hydrolyzates of β-Casein(Food & Nutrition)
- Sodium-23 and Lanthanum-139 Nuclear Magnetic Resonance Studies of Cation Binding to Aqualysin I, a Thermostable Serine Protease(Analytical Chemistry)
- Involvement of Glu-264 and Arg-235 in the Essential Interaction between the Catalytic Imidazole and Substrate for the D-Lactate Dehydrogenase Catalysis
- Expression of Aqualysin I (a Thermophilic Protease) in Soluble Form in Escherichia coli under a Bacteriophage T7 Promoter
- Production of Thermophilic Protease by Clucose-Controlled Fed-Batch Culture of Recombinant Escherichia coli
- Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
- Comparison between the Antigenicity of Native and Unfolded β-Lactoglobulin(Biological Chemistry)
- Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer's Patches
- Characterization and Expression Analysis of the Exopolysaccharide Gene Cluster in Lactobacillus fermentum TDS030603
- Mucosal Immunity in CD4-Deficient Mice : Direct Evidence for Mucosal IgA Antibody Responses
- Saccharomyces cerevisiae and Candida albicans Stimulate Cytokine Secretion from Human Neutrophil-Like HL-60 Cells Differentiated with Retinoic Acid or Dimethylsulfoxide
- Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid
- Comparison of the Immunopotentiating Activity with Structural Characteristics among Water-soluble Polysaccharides Isolated from the Genus Bifidobacterium
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Differentiated implication of Lactobacillus GG and L. gasseri TMC0356 to immune responses of murine Peyer's patch
- Localization of Sequential Antigenic Determinants on Bovine β-Casein with Synthetic Peptides and Antisera from Mouse, Rabbit, and Goat
- Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
- Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
- Identification of Polypeptides Constituting Lactophorin by Monoclonal Antibody to Bovine Milk Lactophorin
- Preparation of Anti-bovine β-Casein Monoclonal Antibody and Analysis of the Interaction between the Antibody and β-Casein Fragment(Biological Chemistry)
- Intestinal Immune System and Prebiotics
- Food Allergy, Oral Tolerance and Immunomodulation-Their Molecular and Cellular Mechanisms
- Emulsifying Properties of a Mixture of Peptides Derived from the Enzymatic Hydrolyzates of Bovine Caseins
- Isolation and Characterization of a Prolidase from Streptococcus cremoris H61
- Purification and Properties of a Dipeptidase from Streptococcus cremoris
- Kinetic Properties of a Dipeptidase from Streptococcus cremoris
- Localization of Allergenic Reactive Sites on Hen Ovomucoid
- The Antigenic Properties of β-Lactoglobulin Examined with Mouse IgE Antibody
- Studies on the Allergenic Structure of Hen Ovomucoid by Chemical and Enzymic Fragmentation