Preparation of Monoclonal Antibody against Bovine β-Lactoglobulin and Its Unique Binding Affinity(Biological Chemistry)
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概要
- 論文の詳細を見る
Five hybridoma cell lines, 21B3, 31A4, 61C1, 61B4, and 62A6 were established and characterized to produce IgG_1 antibodies with specificity for bovine β-lactoglobulin (β-LG) which is a potent milk allergen. A representative monoclonal antibody (MAb) 21B3 had a unique affinity; it bound more strongly to RCM (reduced carboxymethylated) β-LG than to native β-LG. The affinity increased drastically when β-LG was heated at 70℃ or above for 10 min at pH 7.0, while β-LG heated at pH 3.0 did not alter its affinity even when heated at 80℃ for 10 min. An enzymeprobe method suggested that unfolding of β-LG by heating at pH 7.0 seemed to start at 70℃, and β-LG heated at 90℃ and RCM β-LG were extensively unfolded. The results indicated that the phase of unfolding and increase of affinity by heating were oincidental. Finally the relationship of the unique affinity of MAb and the β-LG conformation was discussed.
- 社団法人日本農芸化学会の論文
- 1987-03-22
著者
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山内 清
大阪市大 工
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HATTORI Makoto
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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YAMAUCHI Kunio
Department of Agricultural Chemistry, The University of Tokyo
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KURISAKI Jun-ichi
National Institute of Agrobiological Sciences
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KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
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Ando Osamu
Department Of Agricultural Chemistry The University Of Tokyo
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Hattori M
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Yamauchi K
Univ. Tokyo Tokyo Jpn
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Yamauchi Kunio
Department Of Agricultural Chemistry The University Of Tokyo
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Ando O
Univ. Tokyo Tokyo Jpn
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Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
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KURISAKI Jun-ichi
Department of Agricultural Chemistry, The University of Tokyo
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Hattori Makoto
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Kurisaki J
National Institute Of Agrobiological Sciences
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Hattori Makoto
Department Of Agricultural Chemistry The University Of Tokyo
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KURISAKI Jun-ichi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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