A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase(<Feature> Ceramics for Biomedical Applications in Asian Countries)
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概要
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Collagen/calcium phosphate composite materials were prepared using an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. We prepared a collagen/calcium phosphate composite film by adding sequentially calcium chloride, disodium glycerophosphate and alkaline phosphatase to collagen solution and subsequent drying of the resultant composite suspension in a template. The calcium phosphate contained in the film was determined to be mainly hydroxyapatite from FT-IR and X-ray diffraction analysis. The film little swelled in water to keep its original morphology, while a collagen film became solubilized. This suggests the binding between collagen and calcium phosphate in complex materials. When the preosteoblast cell line, MC3T3-E1 cells were cultured on the film, the film was found to support the osteoblastic differentiation from the measurement of alkaline phosphatase activity, which is an early differentiation marker.
- 社団法人日本セラミックス協会の論文
- 2008-01-01
著者
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TACHIBANA Akira
Department of Biological Science, Faculty of Science, Ibaraki University
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山内 清
大阪市大 工
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Yamauchi Kiyoshi
Department of Biology, Faculty of Science, Shizuoka University
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Tanabe Toshizumi
Department of Bioengineering, Graduate School of Engineering, Osaka City University
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Akira Tachibana
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Department Of Biology Faculty Of Science Osaka City University
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Tachibana Akira
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Yamauchi K
Faculty Of Agriculture Miyazaki University
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Tanabe T
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tanabe Toshizumi
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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TOMOMATSU Ohki
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City Univers
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Tomomatsu Ohki
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Yamauchi K
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Rad. Biol. Canter Kyoto Univ.
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Yamauchi Kiyoshi
Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University
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Tachibana Akira
Rad. Biol. Center Kyoto Univ.
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TACHIBANA Akira
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
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Tanabe Toshizumi
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
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