Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.
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概要
- 論文の詳細を見る
The intergenic region between the hemoglobin (hb) and nucleosome assembly protein-1 (nap-1) genes in the Paramecium caudatum macronuclear genome was previously found to be heterogeneously composed. Cloning of this intergenic region from the macronuclear genomic DNA identified four unique DNA fragments of different sizes. Sequencing of the cloned fragments revealed extreme heterogeneity and characteristics of both internal eliminated sequence (IES) and imprecise internal deletion sequences (IIDSs) in the intergenic region. Missing sequences were an AT-rich and direct repeats existed in their boundaries. Southern blotting of the total genomic DNA and polymerase chain reaction (PCR) of the total genomic DNAs indicated that there exist a dozen DNA fragments of different sizes in this intergenic region. It is likely that the heterogeneity found in the P. caudatum macronuclear genome results from the variable removal of an intergenic region.
- Genetics Society of Japanの論文
- 2010-02-25
著者
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YAMAUCHI Kohei
Graduate School of Fisheries Sciences, Hokkaido University
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Yamauchi K
Graduate School Of Fisheries Sciences Hokkaido University
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Yamauchi K
Hokkaido Univ. Hokkaido Jpn
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Yamauchi K
Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University
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Yamauchi Kohei
Graduate School Of Fisheries Sciences Hokkaido University
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YAMAUCHI KOHEI
The Third Department of Internal Medicine Iwate Medical University School of Medicine
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Mikami Kazuyuki
Environmental Education Center, Miyagi University of Education
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Ochiai Takehiko
Department of Biology, School of Medicine, Fukushima Medical University
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Matsuoka Ariki
Department Of Biology Fukushima Medical University
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Ochiai Takehiko
Department Of Biology Fukushima Medical University School Of Medicine
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Yamauchi K
Third Department Of Internal Medicine Iwate Medical University School Of Medicine
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Yamauchi Kiyoshi
Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University
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Mikami Kazuyuki
Environmental Education Center Miyagi University Of Education
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Mikami K
Environmental Education Center Miyagi University Of Education
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Nishiyama Norihito
Department Of Biological Science Faculty Of Science Shizuoka University
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Ochiai Takehiko
Department Of Biology School Of Medicine Fukushima Medical University
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Matsuoka Ariki
Department Of Biology School Of Medicine Fukushima Medical University
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