Protoplasmic Streaming and Nuclear Differentiation during Conjugation of Paramecium caudatum(Cell Biology)

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概要

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• It is known in Paramecium that the fate of the micronucleus is determined by the intracellular localization of the nucleus and that protoplasmic streaming named cyclosis is very active during vegetative phase. The active streaming, if it occurs during conjugation, may interfere with the correct positioning of micronuclei and/or cytoplasmic determinants in a cell. In the present work, the velocity of protoplasmic streaming was measured during conjugation of P. caudatum. The results showed that the velocity changed remarkably at 3 stages. (1) It increased with the expansion of its active area 3 to 5 minutes after the mating reaction and then decreased again to the ordinary speed within 3 hours. The results of micro- or macronuclear removal showed that the increase in the velocity was not mediated either by the micronucleus or by the macronucleus. Moreover, injection of the anti-α-tubulin antibody at the mating reaction did not suppress the protoplasmic streaming effectively, although a role of microtubules on the protoplasmic streaming is not ruled out completely. (2) At the stages of disintegration of meiotic products and the exchange of gametic nuclei, the cytoplasm ceased streaming and then resumed it soon after synkaryon formation. (3) The streaming ceased again at the critical stage of macro- and micronuclear differentiation after the separation of mating pair, although the streaming was very active before and after this stage. Both cessations of (2) and (3) were not influenced by removal of the micronucleus during conjugation. The velocity of protoplasmic streaming during conjugation does not directly correlated with the micronuclear behavior. The cessation seems to be very convenient for intracellular positioning of the cytoplasmic determinants and/or of the micronuclei for the nuclear differentiation.

• 社団法人日本動物学会の論文
• 1999-12-15

著者

• Mikami Kazuyuki

  Environmental Education Center, Miyagi University of Education

• Mikami Kazuyuki

  EEC, Miyagi University of Education

• Mikami Kazuyuki

  Eec Miyagi University Of Education

• Kobayashi Kumiko

  EEC, Miyagi University of Education

• Yoshimura Chiyo

  EEC, Miyagi University of Education

• Mikami K

  Environmental Education Center Miyagi University Of Education

• Kobayashi Kumiko

  Eec Miyagi University Of Education:(present Address)institute Of Development Aging And Cancer Tohoku University

関連論文

• Phylogenetic Relationships between Vorticella convallaria and Other Species Inferred from Small Subunit rRNA Gene Sequences(Taxonomy)
• Nuclear Behavior and Differentiation in Paramecium caudatum, Analyzed by Immunofluorescence with Anti-tubulin Antibody(Developmental Biology)
• Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.
• MACRONUCLEAR GENOME WAS GENERATED BY TWO DISTINCT DNA-SPLICING MECHANISMS IN PARAMECIUM CAUDATUM(Taxonomy and Systematics & Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• ISOLATION OF MICRONUCLEI AND SEARCHING FOR MICRONUCLEUS-SPECIFIC DNA SEQUENCES IN PARAMECIUM CAUDATUM(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• DNA PROCESSING BETWEEN GENES pap AND histone H4 DURING CUNJUGATION IN Paramecium caudatum(Genetics)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
• The presence of chimeric DNA consisting of 5' regions of the hemoglobin and nucleosome assembly protein-1 genes in Paramecium caudatum macronuclear genomic DNA
• ANALYSIS OF GENES EXPRESSION AT GAMETOGENESIS AND FERTILIZATION IN PARAMECIUM CAUDATUM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Identification of a possible stem-cell-maintenance gene homologue in the unicelluler eukaryote Paramecium caudatum
• ANALYSIS OF GENES EXPRESSED PRIOR TO NUCLEAR DIFFERENTIATION OF PARAMECIUM CAUDATUM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Role of the cytoplasmic and the intranuclear microtubules on the behavior of pronuclei during conjugation in Paramecium caudatum
• Protoplasmic Streaming and Nuclear Differentiation during Conjugation of Paramecium caudatum(Cell Biology)
• DETERMINATION OF A GERMINAL MICRONUCLEUS IN EXCONJUGANTS OF PARAMECIUM CAUDATUM(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• SELECTION OF A GERMINAL MICRONUCLEUS AFTER CONJUGATION OF PARAMECIUM CAUDATUM(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• REJUVENATION OF MATERNAL MACRONUCLEUS AFTER CONJUGANTION OF PARAMECIUM CAUDATUM; ANALYSED BY MACRONUCLEAR TRANSPLANTATION(Developmental Biology & Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.

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