Identification of a possible stem-cell-maintenance gene homologue in the unicelluler eukaryote Paramecium caudatum

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概要

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• In macronuclear DNA of the ciliated protozoan Paramecium caudatum, we identified a gene homologous to aubergine/piwi in Drosophila and ZWILLE in Arabidopsis, which have recently been shown to regulate the maintenance of stem cell in animals and plants. The DNA sequence was considered to be actually transcribed because it was also obtained from cDNA of vegetative phase cells by RACE-PCR. The gene named pap (Paramecium aubergine/piwi homologue) encodes 781 amino acids and contains three short introns (21bp, 22bp and 23bp) in the ORF. Forty four UAA colons and 8 UAG colons which code for glutamine in Paramecium were found in the ORF. This is the first finding of piwi/ZWILLE homologue in a protozoan. The gene was transcribed actively immediately after induction of conjugation, while the transcription was restricted amount during vegetative phase. The result suggests that the gene is integrated in the developmental process of conjugation.

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著者

• Obara Shinji

  Biol. Inst. Grad. Sch. Of Sci. Tohoku Univ.(res. Assist. Of Miyagi Univ. Of Edu.)

• Mikami Kazuyuki

  EEC, Miyagi University of Education

• Iwataki Yoshinori

  Eec Miyagi Univ. Of Edu.

• Iwataki Yoshinori

  EEC, Miyagi Univ. of Edu.

• OBARA Shinji

  Biological Institute, Faculty of Science, Tohoku University

関連論文

• Nuclear Behavior and Differentiation in Paramecium caudatum, Analyzed by Immunofluorescence with Anti-tubulin Antibody(Developmental Biology)
• ISOLATION OF MICRONUCLEI AND SEARCHING FOR MICRONUCLEUS-SPECIFIC DNA SEQUENCES IN PARAMECIUM CAUDATUM(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• DNA PROCESSING BETWEEN GENES pap AND histone H4 DURING CUNJUGATION IN Paramecium caudatum(Genetics)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
• Identification of a possible stem-cell-maintenance gene homologue in the unicelluler eukaryote Paramecium caudatum
• ANALYSIS OF GENES EXPRESSION AT GAMETOGENESIS AND FERTILIZATION IN PARAMECIUM CAUDATUM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Identification of a possible stem-cell-maintenance gene homologue in the unicelluler eukaryote Paramecium caudatum
• ANALYSIS OF GENES EXPRESSED PRIOR TO NUCLEAR DIFFERENTIATION OF PARAMECIUM CAUDATUM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Role of the cytoplasmic and the intranuclear microtubules on the behavior of pronuclei during conjugation in Paramecium caudatum
• Protoplasmic Streaming and Nuclear Differentiation during Conjugation of Paramecium caudatum(Cell Biology)
• REJUVENATION OF MATERNAL MACRONUCLEUS AFTER CONJUGANTION OF PARAMECIUM CAUDATUM; ANALYSED BY MACRONUCLEAR TRANSPLANTATION(Developmental Biology & Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)

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