Nuclear Behavior and Differentiation in Paramecium caudatum, Analyzed by Immunofluorescence with Anti-tubulin Antibody(Developmental Biology)

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To study the dynamics of the microtubules throughout the sexual cycle of Paramecium caudatum, we employed a monoclonal antibody (mAb) N356, that specifically recognized the 50-57 kDa axonemal proteins, equivalent to the tubulins of P. caudatum. The mAb decoration was observed on the micronuclei, as well as structures that consisted of microtubules not only in P. caudatum but also in other species P. bursaria, P. tetraurelia and P. trichium. While the micronucleus was consistently decorated with mAb, the macronucleus was decorated only during morphological changes, that is, at binary fission of the vegetative phase and at skein-formation during conjugation. During conjugation, the oral apparatus, with its associated microtubular structures, disappeared at meiosis I then reappeared at the end of the third division of the synkaryon. The current study led to several major findings on the behavior of the germinal micronucleus during conjugation : 1) the microtubules seemed to connect some of the haploid nuclei to the paroral region at the end of meiosis II ; 2) mAb decoration disappeared from degenerating haploid nuclei, while it remained in the surviving nucleus ; 3) mAb decoration also disappeared from macronuclear anlagen after the initiation of nuclear differentiation, while it remained in the other four post-zygotic micronuclei. The mAb decoration pattern could be used as an indicator of the fate of a micronucleus and suggests that both the intra-micronuclear and the cytoplasmic microtubules might have an important role in determining of the fate of micronuclei by translocating them to specific areas of the cytoplasm.
社団法人日本動物学会の論文
1999-12-15

Nuclear Behavior and Differentiation in Paramecium caudatum, Analyzed by Immunofluorescence with Anti-tubulin Antibody(Developmental Biology)

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