Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1) : Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexua

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概要

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• Cholesterol side chain cleavage cytochrome P450 (CYP11A1: P450scc) is a crucialsteroidogenic enzyme that catalyzes an initial step in the production of all classes ofsteroids. A cDNA encoding Japanese eel P450scc was cloned and characterized. ThecDNA putatively encoded 521 amino acid residues with high homology to those of othervertebrate forms. The recombinant P450scc produced in COS-7 cells efficientlycatalyzed the conversion of 25-hydroxycholesterol into pregnenolone. By northern blot,a single P450scc transcript of approximately 3.3 kb was detected in both ovary and headkidney. Transcript levels of this enzyme significantly increased throughout ovariandevelopment artificially induced by salmon pituitary homogenate, which suggests thatgonadotropic stimuli can induce ovarian expression of the P450scc gene in teleosts, ashas been reported in mammals. Furthermore, RT-PCR analysis revealed that geneexpression of three steroidogenic enzymes, P450scc, P450c17 and 3β-hydroxysteroiddehydrogenase (3β-HSD) show distinctly different tissue specific patterns of expressionin the Japanese eel. The P450scc gene was expressed in ovary and head kidney while thesole source of the P450c17 transcript was ovary. In contrast, 3β-HSD transcript wasdetected in all tissues examined, brain, liver, spleen and trunk kidney, etc. These suggestthat some steroidogenic enzymes are also expressed in non-endocrine tissues and couldpotentially regulate the local and/or circulating steroid levels in teleosts, as they do inmammals.

• Elsevierの論文

著者

• ADACHI SHINJI

  Graduate School of Fisheries Sciences, Hokkaido University

• 井尻 成保

  自然科学研究機構・基礎生物学研究所・生殖生物学研究部門

• IJIRI Shigeho

  Graduate School of Fisheries Sciences, Hokkaido University

• YAMAUCHI Kohei

  Graduate School of Fisheries Sciences, Hokkaido University

• Yamauchi K

  Graduate School Of Fisheries Sciences Hokkaido University

• Yamauchi K

  Hokkaido Univ. Hokkaido Jpn

• Yamauchi K

  Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University

• Yamauchi Kohei

  Graduate School Of Fisheries Sciences Hokkaido University

• Ijiri Shigeho

  Graduate School Of Fisheries Sciences Hokkaido University

• Adachi S

  Graduate School Of Fisheries Sciences Hokkaido University

• Adachi Shinji

  Department Of Biology Faculty Of Fisheries Hokkaido University

• YAMAUCHI KOHEI

  The Third Department of Internal Medicine Iwate Medical University School of Medicine

• Adachi S

  Graduate School Of Fisheries Science Hokkaido University

• Yamauchi K

  Third Department Of Internal Medicine Iwate Medical University School Of Medicine

• Koya Yasunori

  Hokkaido National Fisheries Research Institute

• Adachi Shinji

  Faculty Of Fisheries Hokkaido University

• Ijiri Shigeho

  Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University

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