Specific Antibodies against Synthetic Peptides of Salmonid GTH I and II β Subunits
スポンサーリンク
概要
- 論文の詳細を見る
Specific polyclonal antibodies against the two salmonid gonadotropins (GTHs) were developed using two synthetic oligopeptides corresponding to GTH Iβ-(96-113) and GTH IIβ-(107-119), respectively. By Western blot analysis under reducing conditions, anti-GTH Iβ-(96-113) detected one specific band corresponding to GTH Iβ(17 kDa) and anti-GTH IIβ-(107-119) detected one specific band corresponding to GTH IIβ (20 kDa). Neither antibody recognized bands under nonreducing conditions. By immunohistochemical analysis, immunoreactive (ir-) Iβ-(96-113)-and ir-IIβ-(107-119)-cells had different regional distributions among the aldehyde fuchsin positive cells and were not stained with an anti-human thyrotropin β antibody. In salmonid fishes (chum salmon, rainbow trout, brook trout, whitespotted char, dolly varden and huchen), each antibody reacted with distinctly separate hypophyseal cells. However, in nonsalmonid species, and even in the salmoniform fish, the ayu (Plecoglossus altivelis), no pituitary cell was stained with these antibodies. These data indicate that these antibodies are useful tools for studying the change and the function of GTHs in salmonid fishes.
- 社団法人日本動物学会の論文
- 1995-06-15
著者
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MOCHIDA Kazuhiko
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency
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Ura Kazuhiro
Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University
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Ura Kazuhiro
Laboratory Of Fresh-water Fish-culture Faculty Of Fisheries Hokkaido University
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Ura Kazuhiro
Department Of Biological Sciences Faculty Of Fisheries Hokkaido University
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YAMAUCHI Kohei
Graduate School of Fisheries Sciences, Hokkaido University
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Yamauchi K
Hokkaido Univ. Hokkaido Jpn
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Adachi S
Graduate School Of Fisheries Sciences Hokkaido University
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Adachi Shinji
Department Of Biology Faculty Of Fisheries Hokkaido University
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Yamauchi Kohei
Department Of Biology Faculty Of Fisheries Hokkaido University
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Ikeuchi Toshitaka
Department of Biology, Faculty of Fisheries, Hokkaido University
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Mochida Kazuhiko
Department of Biology, Faculty of Fisheries, Hokkaido University
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Ikeuchi Toshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Adachi S
Graduate School Of Fisheries Science Hokkaido University
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Mochida Kazuhiko
Department Of Biology Faculty Of Fisheries Hokkaido University
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Ikeuchi Toshitaka
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Yamauchi Kohei
Department of Biological Science, Faculty of Science, Shizuoka University
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