Comparative Studies between in vivo and in vitro Spermatogenesis of Japanese Eel (Anguilla japonica)(Reproductive Biology)
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概要
- 論文の詳細を見る
In order to check the quality of in vitro spermatogenesis of Japanese eel, in vitro11-ketotestosterone (11-KT) induced spermatogenesis was compared with in vivo spermatogenesis induced by a single injection of human chorionic gonadotropin (hCG) in detail. DNA contents of germ cells from in vitro and in vivo testicular fragments were compared using flow cytometry. Since the in vitro result of flow cytometry showed prominent 1C peak including spermatozoa and spermatids, the reduction of DNA by meiosis was assumed to progress normally, (i. e., haploid spermatozoa were produced in this in vitro system). In the testes of in vitro culture, however, spermatozoa were not released into lumen. Furthermore, the number of mitotic divisions of the in vitro experiment (6 divisions) was fewer than that of in vivo (10 divisions). In electron microscopy observations, both of in vivo and in vitro spermatozoon had a crescent-shaped nucleus with a flagellum, and a single large spherical mitochondrion. However, the elongation of the sperm head was not sufficient and the mitochondrion was not always located at the anterior end as is observed for the spermatozoa obtained from hCG injected eels. Eel spermatogenesis related substance-11 (eSRS11) is homologue of histone H1 which is up-regulated during spermatogenesis. Using this probe, in vitro spermatogenesis was also evaluated in molecular levels. In Northern blot analysis, eSRS11 mRNA was detected in both in vivo and in vitro testes. However, the expression of in vitro was much weaker than that of in vivo. These differences indicate that the stimulation of 11-KT is not sufficient, and another factors are needed to induce complete spermatogenesis in vitro.
- 社団法人日本動物学会の論文
- 2002-03-25
著者
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三浦 徹
北大院・地球環境
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YAMAUCHI Kohei
Graduate School of Fisheries Sciences, Hokkaido University
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Yamauchi K
Graduate School Of Fisheries Sciences Hokkaido University
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Yamauchi K
Hokkaido Univ. Hokkaido Jpn
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Yamauchi K
Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University
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Yamauchi Kohei
Graduate School Of Fisheries Sciences Hokkaido University
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Yamauchi Kohei
Division Of Marine Biosciences Graduate School Of Fisheries Science Hokkaido University
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Miura C
Jst‐presto Jpn
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YAMAUCHI KOHEI
The Third Department of Internal Medicine Iwate Medical University School of Medicine
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Miura Chiemi
Department of Agrobiological Science, Faculty of Agriculture, Ehime University
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Miura Chiemi
Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University
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Miura Takeshi
Marine Bioresources Research Group, Field Science Center for Northern Biosphere, Hokkaido University
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Ando Nobutaka
Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University
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Miura Takeshi
High Frequency And Optical Device Works Mitsubishi Electric Corporation
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Miura Takeshi
Marine Bioresources Research Group Field Science Center For Northern Biosphere Hokkaido University
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Ando Nobutaka
Division Of Marine Biosciences Graduate School Of Fisheries Science Hokkaido University
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Yamauchi K
Third Department Of Internal Medicine Iwate Medical University School Of Medicine
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Miura C
Division Of Marine Biosciences Graduate School Of Fisheries Science Hokkaido University
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Miura Chiemi
Division Of Marine Biosciences Graduate School Of Fisheries Science Hokkaido University
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