Preparation of long sticky ends for universal ligation-independent cloning : Sequential T4 DNA polymerase treatments(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
Ligation-independent cloning (LIC) is a useful method for efficient directional cloning of a PCR product. LIC requires a specially designed vector containing a long stretch of sequence that is missing any one of the four nucleotides. When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning. In this study, long and efficient sticky ends for LIC were produced by sequential T4 DNA polymerase treatments at non-specific sequences on a commercially available vector. All restriction enzyme sites become available in the current LIC.
- 社団法人日本生物工学会の論文
著者
-
UENO Takayuki
Department of Thoracic and Cardiovascular Surgery, Graduate School of Medicine, Kagoshima University
-
TACHIBANA Akira
Department of Biological Science, Faculty of Science, Ibaraki University
-
Ueno Takayuki
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Harada Ayako
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Tohiguchi Kazuo
Department of Bioengineering, Graduate School of Engineering, Osaka City University
-
Setogawa Yuichi
Department of Bioengineering, Graduate School of Engineering, Osaka City University
-
Tanabe Toshizumi
Department of Bioengineering, Graduate School of Engineering, Osaka City University
-
Tachibana Akira
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Tanabe Toshizumi
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Tohiguchi Kazuo
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Setogawa Yuichi
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
TACHIBANA Akira
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
-
Tanabe Toshizumi
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
関連論文
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- Two Major Factors Involved in the Reverse Dose-rate Effect for Somatic Mutation Induction are the Cell Cycle Position and LET Value
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- Left Ventricular Reconstruction with or without Mitral Annuloplasty
- Impact of Subvalvular Procedure for Ischemic Mitral Regurgitation on Leaflet Configuration, Mobility, and Recurrence
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- Intraoperative infusion of epoprostenol sodium for patients with heparin-induced thrombocytopenia undergoing cardiac surgery
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- PJ-605 Mechanism of Late-Onset Ischemic Mitral Regurgitation Following Dor's Plasty : Potential Relation to Increased Left Ventricular Filling Pressure Inducing Later Remodeling.(Valvular Heart Disease/Pericarditis/Cardiac Tumor 3 (M) : PJ102)(Poster Sess
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- PJ-605 Mechanism of Late-Onset Ischemic Mitral Regurgitation Following Dor's Plasty : Potential Relation to Increased Left Ventricular Filling Pressure Inducing Later Remodeling.(Valvular Heart Disease/Pericarditis/Cardiac Tumor 3 (M) : PJ102)(Poster Sess
- Significance of Membrane Type 1 Matrix Metalloproteinase Expression in Breast Cancer
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- Transient late-onset ischemic mitral regurgitation following Dor's procedure
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- 5 Subvalvular Procedure for Ischemic Mitral Regurgitation improved Anterior Leaflet Configuration and Motion with Reduction of Recurrence(Symposium 9 (SY-09) (M) New Insight for Treatment of Ischemic Mitral Regurgitation,Special Program,The 72nd Annual Sc
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- PJ-285 Diastolic Mitral Valve Area is Smaller Than Annular Area in Patients with Surgical Annuloplasty(Vascular heart disease/Pericarditis/Cardiac tumor-4 (M) PJ48,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Ci
- PJ-605 Mechanism of Late-Onset Ischemic Mitral Regurgitation Following Dor's Plasty : Potential Relation to Increased Left Ventricular Filling Pressure Inducing Later Remodeling.(Valvular Heart Disease/Pericarditis/Cardiac Tumor 3 (M) : PJ102)(Poster Sess
- PJ-605 Mechanism of Late-Onset Ischemic Mitral Regurgitation Following Dor's Plasty : Potential Relation to Increased Left Ventricular Filling Pressure Inducing Later Remodeling.(Valvular Heart Disease/Pericarditis/Cardiac Tumor 3 (M) : PJ102)(Poster Sess
- A Monoclonal Antibody, 3A10, Recognizes a Specific Amino Acid Sequence Present on a Series of Developmentally Expressed Brain Proteins
- Preparation of long sticky ends for universal ligation-independent cloning : Sequential T4 DNA polymerase treatments(METHODS)
- Modified Keratin Sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation(MEDICAL BIOTECHNOLOGY)
- A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase( Ceramics for Biomedical Applications in Asian Countries)
- Drug Release from Hydrogel Containing Albumin as Crosslinker(MEDICAL BIOTECHNOLOGY)
- Keratin Sponge: Immobilization of Lysozyme(MEDICAL BIOTECHNOLOGY)
- Purification and Characterization of Geranylgeranyl Diphosphate Synthase from Methanobacterium thermoformicicum SF-4
- Some Properties of F_-Reducing Hydrogenase of Methanobacterium thermoformicicum Strain SF-4
- Purification and Some Properties of Aspartate Aminotransferase of Methanobacterium thermoformicicum SF-4(Biological Chemistry)
- Use of Continuous Retrograde Gaseous Oxygen Persufflation for Myocardial Protection During Open Heart Surgery
- Electroluminescence in Perylene-Doped Anthracene Films : The Ambient Gas Effect in the Emission Process
- Ki67 index changes, pathological response and clinical benefits in primary breast cancer patients treated with 24 weeks of aromatase inhibition
- Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells
- Prognostic significance of tumor/stromal caveolin-1 expression in breast cancer patients
- Breast MR Image Fusion by Deformable Implicit Polynomial (DIP)
- Breast MR Image Fusion by Deformable Implicit Polynomial (DIP)
- The methylation of ribonucleosides by trimethyl phosphate or dimethyl sulfate in the presence of boric acid.
- Methylation of deoxynucleotides with trimethyl phosphate in aqueous phase.
- Methylation of pyrimidine 2'-deoxynucleosides with trimethyl phosphate.
- The methylation of nucleic acids. The analysis of methylated ribonucleosides by means of high-performance liquid ODS-silica gel and cation-exchange chromatography.
- Allylation of nucleic acid-bases with triallyl phosphate.
- The ethylation of nucleic acid-bases with triethyl phosphate.
- Personality and Resilience Associated with Perceived Fatigue of Local Government Employees Responding to Disasters