Drug Release from Hydrogel Containing Albumin as Crosslinker(MEDICAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Albumin is the major plasma protein and acts as a physiological carrier for various compounds including drugs. To take advantage of the drug-binding ability of albumin for a drug delivery system, we have prepared hydrogels consisting of acrylamide (AAm) and bovine serum albumin (BSA) by introducing three to four vinyl groups into one BSA molecule and subsequently copolymerizing it with AAm. The resultant hydrogel was solubilized by trypsin treatment, since BSA served as a crosslinker in the hydrogel. The BSA-crosslinked hydrogel (BSA-AAm hydrogel) was loaded with salicylic acid or sodium benzoate and their release was investigated. The BSA-AAm hydrogel released much more salicylic acid than sodium benzoate. In addition, the amount of released salicylic acid increased with the BSA content of the hydrogel, despite a decrease in the swelling ratio of the hydrogel. On the other hand, the amount of released sodium benzoate increased with the swelling ratio. When a hydrogel crosslinked with N, N'-methylenebis (acrylamide) was used as a control, both drugs showed release tendencies similar to that of sodium benzoate from the BSA-AAm hydrogel. Furthermore, the salicylic acid release was sustained longer on the BSA-AAm hydrogel than the sodium benzoate release. Taken together, it is thought that albumin in the BSA-AAm hydrogel preferentially adsorbs salicylic acid and contributes to the high drug loading and the sustained release of salicylic acid.
- 社団法人日本生物工学会の論文
- 2005-11-25
著者
-
TACHIBANA Akira
Department of Biological Science, Faculty of Science, Ibaraki University
-
山内 清
大阪市大 工
-
Yamauchi Kiyoshi
Department of Biology, Faculty of Science, Shizuoka University
-
Tanabe Toshizumi
Department of Bioengineering, Graduate School of Engineering, Osaka City University
-
Akira Tachibana
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
-
Tachibana Akira
Department Of Biology Faculty Of Science Osaka City University
-
Tachibana Akira
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Yamauchi K
Faculty Of Agriculture Miyazaki University
-
Tanabe T
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
-
Tanabe Toshizumi
Department Of Bioengineering Graduate School Of Engineering Osaka City University
-
Tada Daisuke
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City Universit
-
Tada Daisuke
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
-
Yamauchi K
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
-
Tachibana Akira
Rad. Biol. Canter Kyoto Univ.
-
Yamauchi Kiyoshi
Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University
-
Tachibana Akira
Rad. Biol. Center Kyoto Univ.
-
TACHIBANA Akira
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
-
Tanabe Toshizumi
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
関連論文
- Two Major Factors Involved in the Reverse Dose-rate Effect for Somatic Mutation Induction are the Cell Cycle Position and LET Value
- Relationship between cell killing and mutation induction by ionizing radiation in human lymphoblastoid cells
- Development, Beam Characterization and Chromosomal Effectiveness of X-rays of RBC Characteristic X-ray Generator
- Mutations and sequence variability of FAA gene in Japanese Fanconi anemia
- Signaling and Gene Regulation of Adaptive Response to Low-dose Radiations
- Cellular Response to Low-dose Radiations and Its Signal Transduction
- DNA Fluorescence in situ Hybridization in Paramecium: Telomere Localization in Macronucleus : Developmental Biology
- Biological Effectiveness of Ultrasoft X-rays from the Characteristic X-ray Generator of Radiation Biology Center, Kyoto University
- EXPRESSION REGULATION OF PROHORMONE CONVERTASES, PC1 AND PC2, BY CORTICOSTERONE(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Novel mutations of the FANCG gene causing alternative splicing in Japanese Fanconi anemia
- Synthesis of Fluorescent Peptides and Application for β-Structural Peptide
- Amphiphilic Peptides from pH-Dependent β-Structure in Phospholipid Membrane
- Analysis of Junction Sequences of Double-Strand Breaks in Plasmid DNA by Nuclear Extracts from X-Irradiated Mouse Cells
- Analysis of Mutations at the FANCC and FANCG genes in Japanese Fanconi anemia patients
- DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
- DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
- Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
- Preparation of long sticky ends for universal ligation-independent cloning : Sequential T4 DNA polymerase treatments(METHODS)
- Effects of α-Tocopherol Levels in Extrusion Pellets on In vivo Lipid Peroxidation Levels and Antioxidant Activities in Cultured Yellowtail Seriola quinqueradiata Injected with the Causative Bacteria of Fish Jaundice
- 古細菌脂質膜モデルと機能
- 人工細胞膜を工夫する(話題を探る)
- C-Pペプチド (C-P化合物の代謝と機能)
- In vivo Lipid Peroxidation Levels and Antioxidant Activities of Cultured and Wild Yellowtail
- Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography
- Occurrence of Hyperbilirubinemia in Cultured Red Sea Bream
- 2-Thiobarbituric Acid Values and Contents of α-Tocopherol and Bile Pigments in the Liver and Muscle of Jaundiced Yellowtail, Seriola quinqueradiata(Biological Chemistry)
- Uncompetitive Inhibition of Xenopus laevis Aldehyde Dehydrogenase 1A1 by Divalent Cations(Biochemistry)
- Spectrum of Spontaneous Mutations in the Cyclic AMP Receptor Protein Gene on Chromosomal DNA of Escherichia coli
- Isolation and Characterization of a β-Primeverosidase Concerned with Alcoholic Aroma Formation in Tea Leaves
- A Primeverosidase as a Main Glycosidase Concerned with the Alcoholic Aroma Formation in Tea Leaves
- Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
- Relationships between 4-Hydroxy-2-nonenal, 2-Thiobarbituric Acid Reactive Substances and n-6 Polyunsaturated Fatty Acids in Refrigerated and Frozen Pork
- A Lipid Peroxidation-derived Aldehyde, 4-Hydroxy-2-nonenal and ω6 Fatty Acids Contents in Meats
- Synthesis and Structural Analysis of The Ligand Binding Site of Macrophage Scavenger Receptor
- Synthesis and Adhesion Activity of a Heterotrimeric Peptide Containing the Cell Adhesion Site of Type I Collagen
- Different inducibility of radiation- or heat-induced p53-dependent apoptosis after acute or chronic irradiation in human squamous cell carcinoma cells
- Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.
- Electron Transfer on Electrode Modified with Keratin Membranes Containing Manganese Porphyrins
- PURIFICATION AND CHARACTERIZATION OF THYROID - HORMONE - BINDING PROTEINS FROM MASU SALMON SERUM : A HOMOLOG OF HIGHER VERTEBRA TETRANSTHYRETIN
- Experimental Analysis on the Involvement of ATM Gene in the Enhanced Radiosensitivity in AT Cells
- Modified Keratin Sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation(MEDICAL BIOTECHNOLOGY)
- HORMONE BINDING AND ALDEHYDEDEHYDROGENASE ACTIVITIES OF XENOPUS CYTOSOLIC THYROID HORMONE BINDING PROTEIN(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase( Ceramics for Biomedical Applications in Asian Countries)
- Drug Release from Hydrogel Containing Albumin as Crosslinker(MEDICAL BIOTECHNOLOGY)
- Low transposition frequency of the medaka fish Tol2 element may be due to extranuclear localization of its transposase
- Keratin Sponge: Immobilization of Lysozyme(MEDICAL BIOTECHNOLOGY)
- The medaka fish Tol2 transposable element can undergo excision in human and mouse cells
- Spectrum of Spontaneous Mutation of E. Ccli Crp Gene
- Purification and Characterization of Geranylgeranyl Diphosphate Synthase from Methanobacterium thermoformicicum SF-4
- Some Properties of F_-Reducing Hydrogenase of Methanobacterium thermoformicicum Strain SF-4
- Purification and Some Properties of Aspartate Aminotransferase of Methanobacterium thermoformicicum SF-4(Biological Chemistry)
- X-ray-induced Transformation and Reversible Suppression of p53 Expression
- Development of Alpha-ray Irradiation System.
- New amphiphiles : 2'3'-Di-O-acyl-5'-nucleotides
- T Cell Response to Hen's Egg Ovomucoid(Biological Chemistry)
- Comparison of Four Distinct Idiotypes of Monoclonal Antibodies against Hen's Egg Ovomucoid(Biological Chemistry)
- EFFECT OF ENDOCRINE DISRUPTING CHEMICALS (EDCS) ON THYROID HORMONE UPTAKE INTO BULLFROG RED BLOOD CELLS(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Evaluation of beef tallow as a natural source of conjugated linoleic acid
- High-pressure Proteolytic Digestion of Food Proteins : Selective Elimination of β-Lactoglobulin in Bovine Milk Whey Concentrate(Food & Nutrition)
- Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
- Occurrence of High Molecular Weight EGF Complexes in Human Milk(Food & Nutrition)
- Preparation of Monoclonal Antibody against Bovine β-Lactoglobulin and Its Unique Binding Affinity(Biological Chemistry)
- Molecular Cloning of Bullfrog (Rana catesbeiana) Ornithine Transcarbamylase and Induction of Its mRNA during Spontaneous Metamorphosis.
- Synthesis and Liposome-Formation of a Lipid Bearing Cell-Adhesion Peptide Sequence
- Effects of dietary supplementation with conjugated linoleic acid on fatty acid composition and lipid oxidation in chicken breast meat
- Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
- THE EFFECT OF ENDOCRINE DISRUPTING CHEMICALS ON THYROID HORMONE BINDING TO JAPANESE QUAIL TRANSTHYRETIN AND THYROID HORMONE RECEPTOR
- ANALYSIS OF XENOPUS LAEVIS TRANSTHYRETIN GENE PROMOTER REGION
- MOLECULAR MECHANISM OF TEMPERATURE-SENSITIVE INHIBITION OF ANURAN METAMORPHOSIS
- Identification of Thyroid Hormone-Responsive Genes in a Chicken Hepatoma Cell Line, LMH(Endocrinology)
- LOW-TEMPERATURE-ARREST OF ANURAN METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes.
- ASSESSING PHENOL COMPOUNDS FOR THYROID DISRUPTING ACTIVITY USING A COMBINATION OF IN VIVO AND IN VITRO ASSAYS IN XENOPUS LAEVIS(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Synthesis and Properties of Dimeric Glycerophospholipid: 2,2'-Dotriacontanedioylbis(1-Palmitoyl-sn-Glycero-3-Phosphocholine)^1
- COMPARISON OF THE EFFECTS OF DISOPYRAMIDE, PROPRANOLOL AND DILTIAZEM ON VENTRICULAR ARRHYTHMIAS : Antiarrhythmic Drugs : IV Auditorium : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- EFFECTS OF ENDOCRINE DISRUPTING CHEMICALS ON THYROID HORMONE BINDING TO TRANSTHYRETINS
- COMPETITIVE INTERACTIONS OF CHLORINATED PHENOLS WITH THYROID HORMONE BINDING TO THYROID-HORMONE-BINDING PROTEINS(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- 1,2-Dotriacontanedioyl-sn-Glycero-3-Phosphocholine : A Cyclic Lipid Used for Phospholipase A_2-Catalvzed Modulation of the Liposome Surface Charge
- Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells
- The methylation of ribonucleosides by trimethyl phosphate or dimethyl sulfate in the presence of boric acid.
- Methylation of deoxynucleotides with trimethyl phosphate in aqueous phase.
- Methylation of pyrimidine 2'-deoxynucleosides with trimethyl phosphate.
- The methylation of nucleic acids. The analysis of methylated ribonucleosides by means of high-performance liquid ODS-silica gel and cation-exchange chromatography.
- Allylation of nucleic acid-bases with triallyl phosphate.
- The ethylation of nucleic acid-bases with triethyl phosphate.
- Quantitative Relationship between Alpha-tocopherol and Polyunsaturated Fatty Acids and Its Connection to Development of Oxidative Rancidity in Chicken Skeletal Muscle
- 1,1'-(1,32-Dotriacontanediyl)bis(2-acetyl-sn-glycero(3)phosphocholine): A long persisting agonist as a potential antihypertension agent.
- Selective 2'-O-methylation of pyrimidine-ribonucleosides by trimethylsulfonium hydroxide in the presence of Mg2+ and Ca2+ ions.
- Vinyl Polymerization. 272. Synthesis and Polymerization of 9-O-Methacryloylquinine
- Archaebacterial Lipids:Surface Pressure-Surface Area Isotherms of 1,1'-(1,32-Dotriacontanediyl)bis(2-((3RS,7R,11R)-3,7,11,15-tetramethylhexadecyl)-sn-glycero-3-phosphocholine).
- N-alkylation of nitrogen heterocyclic compounds with dialkyl phosphites.
- N-alkylation of cytosine and its nucleosides with trialkyl phosphates.
- Esters of phosphorus oxy acids as alkylating agents. IV. N-Alkylation of imidazole and its analogs with alkyl esters of phosphonic and phosphinic acids.
- The Interrelationship between Polyunsaturated Fatty Acids, a- Tocopherol and Glutathione Peroxidase in Chicken and Porcine Skeletal Muscles
- Peptides Containing Aminophosphonic Acids. III. The Synthesis of Tripeptide Analogs Containing Aminomethylphosphonic Acid
- Personality and Resilience Associated with Perceived Fatigue of Local Government Employees Responding to Disasters
- Peptides Containing Aminophosphonic Acids. II. The Synthesis of Tripeptide Analogs