Comparison of Four Distinct Idiotypes of Monoclonal Antibodies against Hen's Egg Ovomucoid(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Two monoclonal antibodies (mAb 41B3 and mAb 51B3), directed against hen's egg ovomucoid (OM) and different from those we previously reported (mAb 23E5 mAb 32A8), were prepared and purified. A competitive radioimmunoassay using ^<125>I-labeled OM showed that both mAb 41B3 and mAb 51B3 could bind all three homologous domains (domains I (DI), II (DII) and III (DIII)) of OM and that they reacted most efficiently with DIII. To analyze the paratope specificities of these four mAbs, a sandwich assay and a competitive radioimmunoassay were done. Only the pair mAb 41B3 and mAb51B3 could not simultaneously bind the OM or domains in the sandwich assay. Only mAb 41B3 inhibited the binding of mAb 51B3 to antigens and vice versa in the competitive radioimmunoassay. these results suggest that mAb 41B3 and mAb 51B3 recognized a closely related site distinct from the epitopes for mAb 23E5 or mAb 32A8. These mAbs may be useful for general studies on epitopes of protein antigens as well as for analyses of the antigenic determinants of OM.
- 社団法人日本農芸化学会の論文
- 1987-11-23
著者
-
山内 清
大阪市大 工
-
ENOMOTO Atsushi
Department of Pathology, Nagoya University Graduate School of Medicine
-
YAMAUCHI Kunio
Department of Agricultural Chemistry, The University of Tokyo
-
KURISAKI Jun-ichi
National Institute of Agrobiological Sciences
-
KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
-
AOKI YOSHIKAZU
Department of Earth and Planetary Sciences, Faculty of Science, Kyushu University
-
Aoki Yoshikazu
Department Of Agricultural Chemistry The University Of Tokyo
-
Yamauchi K
Univ. Tokyo Tokyo Jpn
-
Yamauchi Kunio
Department Of Agricultural Chemistry The University Of Tokyo
-
Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
-
Enomoto Atsushi
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University
-
KURISAKI Jun-ichi
Department of Agricultural Chemistry, The University of Tokyo
-
Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
Kurisaki J
National Institute Of Agrobiological Sciences
-
KURISAKI Jun-ichi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
関連論文
- Analysis of DOK-6 function in downstream signaling of RET in human neuroblastoma cells
- Milk Fat Globule Membrane Proteins in Aseptically Packed Ultra-heat-treated Milk: Changes during Storage
- Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer’s Patches
- Stimulation of the Secretion of Pro-Inflammatory Cytokines by Bifidobacterium Strains
- Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
- Characteristic Intestinal Microflora of Specific Pathogen-Free Mice Bred in Two Different Colonies and their Influence on Postnatal Murine Immunocyte Profiles
- Role of Organic Anion Transporters in the Tubular Transport of Indoxyl Sulfate and the Induction of its Nephrotoxicity.(GENERAL SESSION BY POSTER PRESENTATION)(KIDNEYS AND URINARY EXCRETION SYSTEM)
- RET receptor signaling : Dysfunction in thyroid cancer and Hirschsprung's disease
- Disruption of Microbial Cells by the Flash Discharge of High-pressure Carbon Dioxide
- Synthesis of Fluorescent Peptides and Application for β-Structural Peptide
- Amphiphilic Peptides from pH-Dependent β-Structure in Phospholipid Membrane
- DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
- DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
- Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
- Stimulatory Effect of Human Lactoferrin on DNA Synthesis in BALB/c 3T3 Cells
- サトウキビ抽出液のブタ免疫応答調節能(免疫学)
- OJ-181 A Novel Akt Substrate Girdin is an Essential Regulator of Cytoskeleton and Cell Motility in Endothelial Cells(Regeneration (angiogenesis / myocardial regeneration)-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-097 The Novel Akt Substrate, Girdin, Plays Essential Roles in Signaling Pathways after VEGF-A Stimulation(Molecular biology, vascular-3 (H) PJ17,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Societ
- Increased Expression after X-Irradiation of MUC1 in Cultured Human Colon Carcinoma HT-29 Cells
- Effects of α-Tocopherol Levels in Extrusion Pellets on In vivo Lipid Peroxidation Levels and Antioxidant Activities in Cultured Yellowtail Seriola quinqueradiata Injected with the Causative Bacteria of Fish Jaundice
- 古細菌脂質膜モデルと機能
- 人工細胞膜を工夫する(話題を探る)
- C-Pペプチド (C-P化合物の代謝と機能)
- In vivo Lipid Peroxidation Levels and Antioxidant Activities of Cultured and Wild Yellowtail
- Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography
- Occurrence of Hyperbilirubinemia in Cultured Red Sea Bream
- Role of Olfaction in Food Preference as Evaluated in an Animal Model
- Apoptosis of Antigen-specific T Cells Induced by Oral Administration of Antigen : Comparison of Intestinal and Non-intestinal Immune Organs
- Isolation and Characterization of a β-Primeverosidase Concerned with Alcoholic Aroma Formation in Tea Leaves
- A Primeverosidase as a Main Glycosidase Concerned with the Alcoholic Aroma Formation in Tea Leaves
- Carboxypeptidase Taq, a Thermostable Zinc Enzyme, from Thermus aquaticus YT-1 : Molecular Cloning, Sequencing, and Expression of the Encoding Gene in Escherichia coli
- Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
- Relationships between 4-Hydroxy-2-nonenal, 2-Thiobarbituric Acid Reactive Substances and n-6 Polyunsaturated Fatty Acids in Refrigerated and Frozen Pork
- A Lipid Peroxidation-derived Aldehyde, 4-Hydroxy-2-nonenal and ω6 Fatty Acids Contents in Meats
- Suppression of Collagen-Induced Arthritis in DBA/1J Mice by Preimmunization with House Dust Mite Extract
- IgE-antibody Specificities of the Patients Allergic to Meat Products
- Enhancing Effect of Interleukin-4 on the Secretion of Interferon-γ by α_-Casein-specific CD8^+ T Cells
- α_-Casein-specific CD8^+ T Cell Clone That Inhibits Its Own Interferon-γ Production by Producing Interleukin-10
- Synthesis and Structural Analysis of The Ligand Binding Site of Macrophage Scavenger Receptor
- Synthesis and Adhesion Activity of a Heterotrimeric Peptide Containing the Cell Adhesion Site of Type I Collagen
- Amino Acid Residue Substitution at T-Cell Determinant-flanking Sites in β-Lactoglobulin Modulates Antigen Presentation to T Cells through Subtle Conformational Change(Food & Nutrition Science)
- In Vivo Macrophage-stimulation Activity of the Enzyme-degraded Water-soluble Polysaccharide Fraction from a Marine Alga (Gracilaria verrucosa)
- Macrophage Stimulation Activity of the Polysaccharide Fraction from a Marine Alga (Porphyra yezoensis):Structure-Function Relationships and Improved Solubility
- Activation of Murine Macrophages by Polysaccharide Fractions from Marine Algae (Porphyra yezoensis)
- Antigen Feeding Increases Frequency and Antigen-specific Proliferation Ability of Intraepithelial CD4^+ T Cells in αβ T Cell Receptor Transgenic Mice(Food & Nutrition Science)
- Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells(Food & Nutrition Science)
- Characterization of a Water-soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4
- Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
- Strong Influence of the Processing of the Antigen on Negative Effects on T Cell Activation by Regions Outside the Determinant Area of Bovine α_-Casein
- Production and Characterization of Anti-human Insulin Antibodies in the Hen's Egg(Biological Chemistry)
- Structure of α_-Casein at an Oil/Water Interface: An Analytical Approach Using Immunochemical Methods(Biological Chemistry)
- Methionine Oxidation and Proline cis-trans Isomerization in α_-Casein on Emulsification with Oxidized Lipid(Biological Chemistry)
- Effect of Orally Administered Non-Viable Lactobacillus Cells on Murine Humoral Immune Responses
- Cytokine Production by the Murine Macrophage Cell Line J774.1 after Exposure to Lactobacilli(Microbiology & Fermentation Technology)
- Adhesion of Lactic Acid Bacteria to Caco-2 Cells and Their Effect on Cytokine Secretion
- In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine
- Analysis of Cytokine Producing Activity of Intestinal Intraepithelial T Cells from TCR β-Chain and δ-Chain Mutant Mice
- Dietary Nucleotides Increase the Proportion of a TCRγδ^+ Subset of Intraepithelial Lymphocytes (IEL) AND IL-7 Production by Intestinal Epithelial Cells (IEC) : Implications for Modification of Cellular and Molecular Cross-talk between IEL and IEC by Dieta
- T Cell Recognition of β-Lactoglobulin(Biological Chemistry)
- Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae(Food & Nutrition)
- Interactions of Human-and Rat-Organic Anion Transporters With Pravastatin and Cimetidine
- Expression of Ret finger protein correlates with outcomes in endometrial cancer
- Girding for migratory cues : roles of the Akt substrate Girdin in cancer progression and angiogenesis
- Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
- Food allergens and mucosal immune systems with special reference to recognition of food allergens by gut-associated lymphoid tissue
- Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
- Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
- Comparison of pH and Ionic Strength Dependence of Interactions between Monoclonal Antibodies and Bovineβ-Lactoglobulin
- Preliminary Human Study for Possible Alteration of Serum Immunoglobulin E Production in Perennial Allergic Rhinitis with Fermented Milk Prepared with Lactobacillus gasseri TMC0356
- Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells
- Modified Keratin Sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation(MEDICAL BIOTECHNOLOGY)
- A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase( Ceramics for Biomedical Applications in Asian Countries)
- Drug Release from Hydrogel Containing Albumin as Crosslinker(MEDICAL BIOTECHNOLOGY)
- Keratin Sponge: Immobilization of Lysozyme(MEDICAL BIOTECHNOLOGY)
- CD109 expression in basal-like breast carcinoma
- Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
- T Cell Response to Hen's Egg Ovomucoid(Biological Chemistry)
- Comparison of Four Distinct Idiotypes of Monoclonal Antibodies against Hen's Egg Ovomucoid(Biological Chemistry)
- Suppression of the Systemic Immune Response to Casein by Oral Administration of a Tryptic Digest of Casein
- High-performance liquid chromatographic determination of lipoamidase (lipoyl-X hydrolase) activity with a novel substrate, lipoyl-6-aminoquinoline
- New Lactococcus Strain with Immunomodulatory Activity : Enhancement of Th1-Type Immune Response
- High-pressure Proteolytic Digestion of Food Proteins : Selective Elimination of β-Lactoglobulin in Bovine Milk Whey Concentrate(Food & Nutrition)
- Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
- Occurrence of High Molecular Weight EGF Complexes in Human Milk(Food & Nutrition)
- Lipid Composition of Plasma Membranes Isolated from Lactating Bovine Mammary Gland(Biological Chemistry)
- Preparation of Monoclonal Antibody against Bovine β-Lactoglobulin and Its Unique Binding Affinity(Biological Chemistry)
- Emulsifying Properties of Peptides Obtained from the Hydrolyzates of β-Casein(Food & Nutrition)
- Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
- Comparison between the Antigenicity of Native and Unfolded β-Lactoglobulin(Biological Chemistry)
- Effect of Bound Phosphate on the Calcium-binding Ability and Calcium-dependent Precipitability of Human β-Casein(Food & Nutrition)
- Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer's Patches
- Synthesis and Liposome-Formation of a Lipid Bearing Cell-Adhesion Peptide Sequence
- Inactivation of Food Microorganisms by High-pressure Carbon Dioxide Treatment with or without Explosive Decompression
- Lethal Effect of High-Pressure Carbon Dioxide on a Bacterial Spore
- Roles of organic anion transporters (OATs) and a urate transporter (URAT1) in the pathophysiology of human disease
- Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
- Preparation of Anti-bovine β-Casein Monoclonal Antibody and Analysis of the Interaction between the Antibody and β-Casein Fragment(Biological Chemistry)
- Synthesis and Properties of Dimeric Glycerophospholipid: 2,2'-Dotriacontanedioylbis(1-Palmitoyl-sn-Glycero-3-Phosphocholine)^1
- 1,2-Dotriacontanedioyl-sn-Glycero-3-Phosphocholine : A Cyclic Lipid Used for Phospholipase A_2-Catalvzed Modulation of the Liposome Surface Charge
- Effect of Whey Hydrolysate Formula on the Transfer of β-Lactoglobulin into Serum and Milk in Mice