Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
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概要
- 論文の詳細を見る
Studies of the physiological functions of intestinal epithelial cells (IECs) have been limited by the difficulty of primary culture of IEC. We established a method for primary culture of mouse IEC by culturing fragments of fetal small intestines pretreated with EDTA. This method reproducibly resulted in the expansion of cytokeratin-positive epithelial cells, and vigorous expansion of the epithelial cells was observed only from intestinal fragments of embryonic days 15-16. These cells expressed alkaline phosphatase activity and major histocompatibility complex (MHC) class II molecules, indicating the mature phenotype of IEC in a small intestine. The cells also presented antigens to CD4+ T cells. Furthermore, the cells expressed various cytokines and chemokines, and the expression was enhanced by bacterial stimulation. These results indicate that the primary-cultured mouse IEC prepared by the method established here can be a beneficial tool in study of the functions of IECs, especially in mucosal immunity.
- 社団法人 日本農芸化学会の論文
著者
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SATO KANAKO
Department of Anesthesiology and Pain Relief Center, The University of Tokyo Hospital
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Yamada Kiyoshi
Department of Microbiology, Nihon University School of Dentistry, Japan
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KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
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TOTSUKA Mamoru
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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MORISHITA Satoru
Department of Applied Biological Chemistry, The University of Tokyo
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