Permeation of Disaccharides Derived from Chohdroitin Sulfate through Human Intestinal Caco-2 Cell Monolayers via the Paracelularl Pathway
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概要
- 論文の詳細を見る
The intestinal absorption of chondroitin sulfate (CS) and its hydrolysate, particularly the disaccharides (Di-CS), was investigated by using human intestinal epithelial Caco-2 cell monolayers. Although the transepithelial transport of CS was not detectable, dose- and time-dependent transport of Di-CS was observed. The transport of Di-CS was proved to be energy-independent and its permeability increased inversely with the transepithelial electrical resistance (TER) value of the Caco-2 cell monolayers. The transport rate for Di-CS was also increased by treating the cell monolayers with such tight junction-opening agents as interferon-γ. These results suggest that Di-CS permeated across the Caco-2 cell monolayers mainly via the paracellular pathway. The permeability of Caco-2 cell monolayers to authentic chondroitin 4-sulfate disaccharides (Di-4S), chondroitin 6-sulfate disaccharides (Di-6S) and chondroitin 0-sulfate disaccharides (Di-0S) was almost same, suggesting that sulfation did not affect the transport rate of Di-CS.
- 社団法人 日本農芸化学会の論文
- 2010-06-23
著者
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SHIMIZU Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Satsu Hideo
Department Of Applied Biological Chemistry Division Of Agricultural And Life Sciences The University
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Yamada Kiyoshi
Department of Microbiology, Nihon University School of Dentistry, Japan
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Satsu Hideo
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Satsu H
Univ. Tokyo Tokyo Jpn
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Totsuka Mamoru
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Totsuka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Totsuka Mamoru
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Jin Miao
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Hisada Nozomi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamada Kiyoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Shimizu Makoto
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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