Establishment of Intestinal Epithelial Cell Lines from Adult Mouse Small and Large Intestinal Crypts
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概要
- 論文の詳細を見る
Small and large intestinal epithelial cell (IEC) lines were established from adult murine intestinal crypts. Both established small and large IECs line (named aMoS7 and aMoC1 respectively) expressed epithelial markers. Similarly to IECs isolated from adult mouse intestines, the expression of major histocompatibility complex (MHC) class II molecules was induced by interferon-γ-treatment in both established cell lines. This expression of MHC class II molecules was higher in small intestinal aMoS7 cells than in large intestinal aMoC1 cells. Treatment with lipopolysaccharide and with ligands of Toll-like receptors 1, 2, 3, and 7 induced secretion of interleukin-6 from both adult IEC lines. These results suggest that the aMoS7 and aMoC1 cell lines can serve as useful tools in analyzing the immunological functions of IECs, especially in studying the IEC response to microbial components and its antigen presenting ability.
- 社団法人 日本農芸化学会の論文
- 2011-05-23
著者
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Totsuka Mamoru
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Totsuka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Totsuka Mamoru
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamada Kiyoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Iwamoto Taku
Department Of Applied Biological Chemistry The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Shimizu Makoto
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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