Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers
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概要
- 論文の詳細を見る
The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 °C. The permeability of T84 cell monolayers to β-lactogulobulin (β-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN3), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4.
- 社団法人 日本農芸化学会の論文
- 2009-11-23
著者
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Satsu Hideo
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Satsu Hideo
Department Of Applied Biological Chemistry Division Of Agricultural And Life Sciences The University
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Shimizu Makoto
Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Satsu Hideo
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Satsu H
Univ. Tokyo Tokyo Jpn
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MOCHIZUKI Tetsunosuke
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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TOTSUKA Mamoru
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Totsuka Mamoru
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Totsuka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Mochizuki Tetsunosuke
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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