The Effect of Hyperosmosis on Paracellular Permeability in Caco-2 Cell Monolayers
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概要
- 論文の詳細を見る
The intestinal epithelium is a significant barrier to oral absorption of hydrophilic compounds, and their passage through the intercellular space is restricted by the tight junctions. In this study we found that hyperosmosis is a significant factor altering paracellular transport in Caco-2 cell monolayers. Osmotic regulators, such as sodium chloride, mannitol, and raffinose, decreased transepithelial electrical resistance and enhanced lucifer yellow permeability. The effect of these osmotic regulators on Caco-2 cell monolayers was not likely to be caused by gross cytotoxicity. Although certain amino acids and oligosaccharides have been reported to have specific tight junction-modulating activity, we found that the increased paracellular permeability of Caco-2 monolayers induced by these compounds was at least partly due to the increased osmotic pressure of the test solutions. These findings provide a new potential precaution in the evaluation of paracellular permeability-modulating substances using the Caco-2 cell monolayer system.
- 社団法人 日本農芸化学会の論文
- 2009-02-23
著者
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SHIMIZU Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Inokuchi Hitoshi
Oral Liquid Formulation R&d Laboratories Self Medication Business Taisho Pharmaceutical Co. Ltd.
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Takei Takuto
Oral Liquid Formulation R&d Laboratories Self Medication Business Taisho Pharmaceutical Co. Ltd.
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AIKAWA Katsuyoshi
Oral Liquid Formulation R&D Laboratories, Self Medication Business, Taisho Pharmaceutical Co., Ltd.
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Aikawa Katsuyoshi
Oral Liquid Formulation R&d Laboratories Self Medication Business Taisho Pharmaceutical Co. Ltd.
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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